Rice SPL12 coevolved with GW5 to determine grain shape  

水稻OsSPL12与GW5存在共进化并协同决定粒型

作　　者：Xiao-Fan Zhang Chun-Yan Yang Hong-Xuan Lin Jia-Wei Wang Hong-Wei Xue 张晓凡;杨春艳;林鸿宣;王佳伟;薛红卫(School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China;Joint Center for Single Cell Biology/School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China;National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China)

机构地区：[1]School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China [2]Joint Center for Single Cell Biology/School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China [3]National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China

出　　处：《Science Bulletin》2021年第23期2353-2357,共5页科学通报（英文版）

基　　金：supported by the National Key Research and Development Program of China (2016YFD0100501);the National Transformation Science and Technology Program (2016ZX08001006-009);the ‘‘Ten-Thousand Talent Program”;the Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China;the Collaborative Innovation Center of Crop Stress Biology, Henan Province。

摘　　要：Asian cultivated rice(Oryza sativa L.)is a staple food crop in China.Accordingly,many studies have focused on grain size and weight as a means to in crease rice yield.Two main subgroups of cultivated rice,Indica and Japonica,have evolved independently under natural and human selection,and a key morphological difference between these subgroups is grain shape:slender in Indica rice and ovate in Japonica.Elucidating the genetic basis of grain shape will provide insights into rice evolution and open new avenues for molecular breeding of improved grain shape.粒型是决定水稻产量和稻米品质的关键因素之一.本研究发现水稻转录因子SQUAMOSA promoter-binding protein-like 12 (Os SPL12)与粒型调控关键基因Grain Width 5(GW5)存在协同进化,最终导致籼粳稻的粒型差异.本研究首先通过群体遗传学与功能学的双重验证,表明籼稻Os SPL12转录激活域的一个单核苷酸多态性(SNP)SNP1066能显著改变Os SPL12的转录活性,使籼稻Os SPL12对粒宽起到抑制作用.随后通过幼穗组织的转录组学分析发现,粒宽调控关键基因GW5特异受籼稻Os SPL12调控. GW5基因启动子区域富含SPLs蛋白的识别与结合基序(GTAC),提示籼稻Os SPL12可能通过直接调控GW5表达最终抑制粒宽.进一步结合"3000份水稻基因组"数据分析发现籼稻Os SPL12与籼稻GW5等位基因的组合显著抑制粒宽.此前的研究表明粳稻中GW5的低表达主要是由于其启动子上游约5-kb处存在一段1212-bp的缺失,本研究进一步通过实验证明籼稻Os SPL12与1212-bp以增强子-启动子的方式促进GW5的表达.综上所述,本研究阐明了水稻驯化中Os SPL12与GW5存在共进化并协同决定了水稻粒型,证明了Os SPL12在籼粳稻粒型分化上的重要作用.

关 键 词：粒型 协同进化 水稻基因组 共进化 数据分析 群体遗传学 

分 类 号：S511[农业科学—作物学]