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作 者:单建军[1] 管崇武[1] 宋红桥[1] 刘世晶[1] 洪扬[1] 庄保陆[1] Shan Jianjun;Guan Chongwu;Song Hongqiao;Liu Shijing;Hong Yang;Zhuang Baolu(Fishery Machinery and Instrument Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200092)
机构地区:[1]中国水产科学研究院渔业机械仪器研究所,农业农村部渔业装备与工程技术重点实验室,上海200092
出 处:《中国农学通报》2021年第29期140-145,共6页Chinese Agricultural Science Bulletin
基 金:国家重点研发计划“设施水产养殖智能化精细生产管理技术装备研发”(2017YFD0701705)。
摘 要:采用主成分分析法建立吸鱼泵的损伤综合评价方法,为吸鱼泵的研究与改进提供装备技术参数。使用真空吸鱼泵对鲫鱼进行吸捕试验,获取鱼体损伤数据。通过测定体表损伤面积比率、24 h存活率、红细胞数量、白细胞数量、超氧化物歧化酶(SOD)活力、谷丙转氨酶(ALT)活力、肌酐(Cr)含量等多个关键指标值,采用主成分分析法进行相关性分析,建立损伤综合评价模型。结果显示:采用主成分分析法提取出3个主成分的累积贡献率达到78.232%,可反映出鱼体损伤的大部分情况;损伤评价综合评价模型:F=0.285X_(1)+0.111X_(2)+0.316X_(3)+0.366X_(4)-0.118X_(5)+0.234X_(6),真空吸鱼泵对鱼的体表和内脏无显著损伤情况,综合得分-0.102,与对照组接近,表明真空吸鱼泵对鱼的损伤影响很小。研究表明,SOD活力、白细胞数和体表损伤面积比率是对损伤评价影响较大的数据指标,采用主成分分析法建立的综合损伤评价模型可以作为评价鱼损伤情况的一种可行方法,为吸鱼泵的改进研究提供了有力参考。To provide technical parameters for the research and improvement of fish pump,the damage comprehensive evaluation method of vacuum fish pump was established by means of Principal Component Analysis(PCA).Experiments on Carassius auratus were carried out by vacuum fish pump to obtain fish body damage data.A comprehensive injury evaluation model was established by measuring the injured surface area ratio,24-hour survival rate,red blood cell number,white blood cell number,superoxide dismutase activity,alanine aminotransferase activity and creatinine content.The results showed that the cumulative contribution rate of three principal components extracted by PCA was 78.232%,which could represent most of the fish damage.The comprehensive evaluation model of fish damage by vacuum fish pump was F=0.285 X_(1)+0.111 X_(2)+0.316 X_(3)+0.366 X_(4)-0.118 X_(5)+0.234 X_(6).There was no significant damage to the surface and viscera of fish by vacuum fish pump,and the comprehensive score was-0.102,which was close to that of the control group,indicating that the damage effect of vacuum fish pump on fish was very small.The results of experiments show that SOD activity,white blood cell count and body surface damage area ratio are the data indexes that have great influence on damage evaluation.The comprehensive damage evaluation model established by PCA can be used as a feasible method to evaluate the damage of fish,which provides a powerful reference for the improvement of fish suction pump.
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