谷子抗咪唑啉酮的遗传应用和基因初定位  

Genetic Application and Mapping of Imidazolinone Herbicides Resistant Traits in Foxtail Millet

作　　者：宋慧[1] 王涛 田礼新 张扬[1] 李龙 邢璐[1] 刘金荣[1] 冯佰利[3] Song Hui;Wang Tao;Tian Lixin;Zhang Yang;Li Long;Xing Lu;Liu Jinrong;Feng Baili(Institute of Foxtail Millet Research,Anyang Academy of Agriculture Sciences/Henan Valley Breeding Engineering Research Center,Anyang Henan 455000;College of Biology and Food Engineering,Anyang Institute of Technology,Anyang Henan 455000;College of Agronomy,Northwest A&F University,Yangling Shannxi 712100)

机构地区：[1]安阳市农业科学院谷子研究所/河南省谷子育种工程技术研究中心,河南安阳455000 [2]安阳工学院生物与食品学院,河南安阳455000 [3]西北农林科技大学农学院,陕西杨凌712100

出　　处：《中国农学通报》2021年第28期1-8,共8页Chinese Agricultural Science Bulletin

基　　金：国家重点研发计划“杂粮作物核心资源遗传本地评价和深度解析”(2019YFD1000700和2019YFD1000702-8);现代农业产业技术体系建设专项资金“国家谷子高粱产业技术体系”(CARS-07-13.5-B25);河南省青年人才托举项目“基于流程化的谷子育种表型性状数据采集与管理系统研究”(2021HYTP035);河南省现代农业产业技术体系建设专项资金“河南省甘薯杂粮产业技术体系”(Z2020-14-01)。

摘　　要：探究咪唑啉酮除草剂在谷子应用上的安全剂量和最大剂量,并对抗咪唑啉酮进行基因初定位,以期为抗咪唑啉酮谷子育种的利用提供理论基础。以谷子抗性材料‘安5158’和敏感材料‘豫谷9号’为研究对象,分别对其种子和幼苗喷施不同浓度咪唑啉酮,并选用250 mL/hm^(2)浓度对亲本和BC1群体喷施,结合转录组分析完成抗咪唑啉酮基因的初定位。研究结果表明,在250 mL/hm^(2)浓度下,敏感材料‘豫谷9号’只有14%的种子能正常发芽但无法存活,而抗性材料‘安5158’的种子发芽率和存活率均不受影响;敏感材料‘豫谷9号’幼苗在喷药第7天,250 mL/hm^(2)浓度下的抗逆生理指标(SOD、POD、CAT、MDA)出现明显的变化,并且不同喷药天数之间差异显著,而抗性材料‘安5158’在450 mL/hm^(2)浓度下才表现相似变化趋势。用250 mL/hm^(2)浓度咪唑啉酮对亲本和BC1群体喷施,回交后代出现50%左右死亡率,符合完全显性遗传规律。基于转录组测序分析,谷子抗咪唑啉酮除草剂基因定位在第1条染色体短臂6.8~30.8 Mb区间内,共有9个与抗咪唑啉酮相关的候选基因。本试验确定了谷子喷施咪唑啉酮除草剂的安全剂量是250 mL/hm^(2),验证了其完全显性遗传规律,并完成抗咪唑啉酮相关候选基因的初定位,为抗除草剂分子辅助育种提供理论基础。To provide a theoretical basis for the utilization of imidazolinone resistant foxtail millet breeding, the safe dose and maximum dose of imidazolinone herbicide applied in foxtail millet were investigated, and the resistance genes to imidazolinone were preliminarily mapped. The resistant material‘An 5158’and sensitive material‘Yugu 9’were used as the research objects. The seeds and seedlings of foxtail millet were sprayed with different concentrations of imidazolinone, and the parents and BC1 populations were sprayed with 250 mL/hm^(2),combined with transcriptome analysis to complete the primary mapping of resistance genes to imidazolinone.The results showed that only 14% seeds of sensitive material‘Yugu 9’could germinate, but could not survive at the concentration of 250 mL/hm^(2), while the germination and survival rate of resistant material‘An 5158’were not affected. On the 7 thday of spraying, the stress response physiological indexes(SOD, POD, CAT,MDA) of the sensitive material‘Yugu 9’seedlings showed significant changes at 250 mL/hm^(2), and the difference was significant among different spraying days, while these indexes of resistance material‘An 5158’began to show a similar trend at 450 mL/hm^(2). When the parents and BC1 populations were sprayed with imidazolinone(250 mL/hm^(2)), the mortality rate of the backcross generations was about 50%, which was in line with the rule of complete dominant inheritance. Based on transcriptome sequencing analysis, the resistance genes to imidazolinone herbicide of foxtail millet were located in the range of 6.8-30.8 Mb on the short arm of the 1 st chromosome, and there were 9 candidate genes in this region. Herein, the safe dose of imidazolinone herbicide was determined to be 250 mL/hm^(2) in foxtail millet, and the complete dominant inheritance rule was verified. The preliminary mapping of the candidate genes related to imidazolinone resistance was completed,which could provide a theoretical basis for molecular assisted breeding of herbicide re

关 键 词：谷子 咪唑啉酮 抗除草剂 基因定位 分子辅助育种 

分 类 号：S-3[农业科学]