百香果茎基腐病菌分离鉴定与药剂筛选研究  

Isolation and Identification of the Pathogen Causing Stem Rot Disease in Passiflora edulia and Fungicide Screening

作　　者：邝瑞彬[1] 杨护[1] 杨敏 周陈平 黄炳雄[1] 魏岳荣[1] Kuang Ruibin;Yang Hu;Yang Min;Zhou Chenping;Huang Bingxiong;Wei Yuerong(Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences/Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research,Guangzhou 510640)

机构地区：[1]广东省农业科学院果树研究所/农业农村部南亚热带果树生物学与遗传资源利用重点实验室/广东省热带亚热带果树研究重点实验室,广州510640

出　　处：《中国农学通报》2021年第28期108-114,共7页Chinese Agricultural Science Bulletin

基　　金：广东省农业农村厅项目“广东省现代农业产业技术体系创新团队建设项目(优稀水果产业)”(2021KJ116);广东省农业农村厅乡村振兴战略专项资金(构建现代农业体系)项目“农作物种质资源库(圃)建设与资源收集保存、鉴评”(粤农计[2018]36号);广州市科技局农村科技特派员项目(GDKTP202012)。

摘　　要：为研究百香果产业中多发病害茎基腐病的病原菌鉴定与防治问题,在广东省百香果主产区采集茎基腐病病株,结合形态学观察与分子测序方法鉴定分离病原菌菌株,并对其进行致病性检测,同时检测系列杀菌药剂对病菌生长及其致病性的影响。结果表明,通过形态特征观察、分子生物学鉴定,从百香果茎基腐病病株中分离获得的8个菌株鉴定为腐皮镰刀菌(Fusarium solani)。离体和活体致病力测试结果显示,致病力较高的菌株为FP6、FP7、FP2,其次为FP1、FP3、FP8、FP4,最低为FP5。不同药剂处理对病菌的生长及致病力均产生影响,其中30%苯甲嘧菌酯处理的抑制效果为最佳。研究结果可为后续百香果茎基腐病病理学、百香果抗病育种等研究提供基础。The paper aims to find out the pathogen which causes stem rot disease in passion fruit,and the chemicals to control this disease effectively.Stem rot diseased samples were collected from different Passiflora edulia production fields in Guangdong.The pathogen was isolated and determined by methods of morphological observation and molecular identification.The strains’pathogenicity was measured,and their sensibility to eight fungicides was also detected.Results showed that eight isolated pathogen strains were identified as Fusarium solani.Based on in vivo and in vitro tests with Passiflora edulis,strains of FP6,FP7 and FP2 showed highest pathogenicity,followed by FP1,FP3,FP8 and FP4,and the lowest was FP5.Different fungicides showed effects on pathogen growth rate and pathogenicity,and 30%benazoxystrobin showed the best inhibition effects.This study could lay a material and technology base for further research on pathogenic mechanism of stem rot disease,and disease resistance breeding program of passion fruit.

关 键 词：百香果 茎基腐病 镰刀菌 致病性 杀菌剂 

分 类 号：S482[农业科学—农药学]