我国27个省份牛恶性卡他热病毒抗体的血清学检测  

Serological Investigation of Bovine Malignant Catarrhal Fever in 27 Provinces and Cities of China

作　　者：孙雨[1] 王睿男[1] 李晓霞 孙航 魏巍[1] 刘颖昳[1] 冯冰 陈玲 顾小雪[1] 王传彬[1] SUN Yu;WANG Ruinan;LI Xiaoxia;SUN Hang;WEI Wei;LIU Yingyi;FENG Bing;CHEN Ling;GU Xiaoxue;WANG Chuanbin(China Animal Disease Control Center,Beijing 102600,China)

机构地区：[1]中国动物疫病预防控制中心,北京102600

出　　处：《中国草食动物科学》2021年第6期24-28,共5页China Herbivore Science

基　　金：十三五国家重点研发计划(2016YFD0500900);现代农业人才支撑计划(2130106-024);跨境动物疫病防控机制建设(2130114-FK)。

摘　　要：为摸清我国牛恶性卡他热(BMCF)的流行情况,用间接ELISA(i-ELISA)方法对采自新疆、青海和甘肃等27个省(市)养殖场、屠宰场和散养户的2731份牛血清进行了抗体检测。同时对选用试剂盒检测质控血清的性能、检测分析敏感性、检测方法的交叉反应等指标进行了验证。结果表明,共检测出阳性牛血清样本29份,阴性牛血清2702份,阳性率1.06%;按不同牛血清来源统计,牛恶性卡他热的规模场阳性率0.56%,散养户阳性率1.18%,屠宰场阳性率1.93%;按不同饲养方式统计,牛全舍饲饲养方式的牛恶性卡他热病毒抗体阳性率0.41%,半舍饲饲养方式的牛恶性卡他热病毒抗体阳性率0.35%,放牧饲养方式的牛恶性卡他热病毒抗体阳性率0.35%。说明我国养殖的牛群中存在牛恶性卡他热的感染,但感染流行率较低,个别省(市)呈散发状态。To find out the epidemic situation of bovine malignant catarrhal fever(BMCF)in China,2731 bovine serum samples collected from farms,and free-range farmers in 27 provinces(cities)including Xinjiang,Qinghai,and Gansu were detected by indirect ELISA.At the same time,the performance,sensitivity,and cross-reaction of the kit were verified.The results showed that 29 bovine serum samples were positive and 2702 serum samples were negative,with a positive rate of 1.06%.According to the statistics of different sources of bovine serum,the positive rate was 0.56%in large-scale farms,1.18%in free-range households,and 1.93%in slaughterhouses.According to different feeding methods,the positive rate of bovine malignant catarrhal fever antibody was 0.41%in full house feeding,0.35%in half house feeding,and 0.35%in grazing feeding.In conclusion,there was infection of bovine malignant catarrhal fever in cattle farms in China,but the prevalence rate was low and sporadic in some provinces and cities.

关 键 词：牛恶性卡他热 抗体检测 血清学调查 

分 类 号：S823.7[农业科学—畜牧学]