基于COI基因的光倒刺鲃群体遗传多样性与遗传分化研究  

Genetic Diversity and Genetic Differentiation of Spinibarbus hollandi Based on COI gene

作　　者：李文俊 李强[1] 韩崇 李陇旭 易祖盛[1] 桂林[1] 钟良明 LI Wen-jun;LI Qiang;HAN Chong(School of Life Science,Guangzhou University,Guangzhou,Guangdong 510006)

机构地区：[1]广州大学生命科学学院,广东广州510006 [2]韶关金粤水产科技有限公司,广东韶关512335

出　　处：《安徽农业科学》2021年第22期125-128,共4页Journal of Anhui Agricultural Sciences

基　　金：广州市科技计划项目(201804010486)。

摘　　要：[目的]全面了解光倒刺鲃的遗传多样性和遗传结构,为其种质资源保护和利用提供科学依据。[方法]采用自行设计的引物对9条水系共209尾光倒刺鲃样本的线粒体COI基因序列进行测定与分析,并探讨其遗传结构和遗传多样性水平。[结果]在209条COI基因序列中,共检测到12个单倍型,单倍型多样性为0.801,核苷酸多样性为0.0082。单倍型系统树显示,所有群体聚成2支,东江群体的全部样本及北江、赣江和九龙江水系的部分样本组成Ⅰ支,其余样本组成Ⅱ支。单倍型网络分析显示,东江群体单倍型与北江、赣江和九龙江部分个体关系较近,但与其他个体关系相对较远;珠江水系大部分群体与长江、钱塘江、九龙江大部分群体分布于不同的分支。AMOVA分析表明,光倒刺鲃COI基因序列变异主要来自地理区内群体间,占82.33%。错配分析及中性检验显示,大多数群体相对稳定,未发生过群体扩张。[结论]光倒刺鲃群体的遗传多样性总体偏低,应加强对其种质资源的保护;东江水系与珠江水系其他群体既存在一定隔离,又存在着基因交流;珠江水系群体与长江水系群体间分化明显。[Objective]To understand the genetic diversity and genetic structure of Spinibarbus hollandi more comprehensively,and provide scientific basis for the conservation and utilization of its wild germplasm resources.[Method]The mitochondrial COI gene sequences of 209 S.hollandi samples from 9 rivers were determined and analyzed with designed primers,and its genetic diversity and genetic differentiation level were discussed.[Result]Among 209 COI gene sequences,12 haplotypes were detected,with haplotype diversity of 0.8010 and nucleotide diversity of 0.0082.Phylogenetic tree of haplotypes showed that all haplotypes gathered into two branches(branches 1:all the samples Dongjiang population and some individuals of Beijiang,Ganjiang and Jiulong River).Network analysis of haplotypes showed that haplotype of Dongjiang population was closely related to some individuals in Beijiang,Ganjiang and Jiulong River,but far related to other individuals.The majority of the population of the Pearl River distributed in different branches compared with the majority of the population of the Yangtze River,Qiantang River,and Jiulong River.AMOVA analysis showed that the variation of COI gene sequences of S.hollandi was mainly from within groups,accounting for 82.33%.Mismatch analysis and neutral test showed that most populations were relatively stable without population expansion.[Conclusion]The genetic diversity of S.hollandi population is generally low,so the protection of its germplasm resources should be strengthened.There is not only certain isolation but also gene exchange between Dongjiang population and other populations in the Pearl River.There is obvious differentiation of populations between the Pearl River and the Yangtze River.

关 键 词：光倒刺鲃 COI基因 遗传多样性 遗传分化 

分 类 号：S917.4[农业科学—水产科学]