泡桐花提取物抑菌抗炎活性评价  

Evaluation on the Antibacterial and Anti-inflammatory Activity of Paulownia elongate Flower Extracts

作　　者：徐立丽 李丽妍[2] XU Li-li;LI Li-yan(Anshan Health School,Anshan,Liaoning 114000;Medical School,Huanghe Science and Technology University,Zhengzhou,Henan 450063)

机构地区：[1]鞍山卫生学校,辽宁鞍山114000 [2]黄河科技学院医学院,河南郑州450063

出　　处：《安徽农业科学》2021年第22期170-173,共4页Journal of Anhui Agricultural Sciences

基　　金：河南省高校重点科研项目(21B350001);河南省科技厅科技攻关项目(172102310165)。

摘　　要：[目的]探究泡桐花资源在饲料添加剂中的开发利用。[方法]制备兰考泡桐花不同提取物,采用牛津杯法测定其抑菌活性,采用梯度稀释法确定其最小抑菌浓度。利用二甲苯致小鼠耳肿胀模型和LPS诱导小鼠巨噬细胞RAW264.7炎症模型,评价泡桐花提取物抗炎活性。[结果]泡桐花水提物对金黄色葡萄球菌、四联球菌、大肠杆菌和沙门氏菌的抑制作用最为显著,其醇提物对白色念珠菌具有一定的抑制作用。泡桐花水提物的抗炎作用最佳,在试验浓度范围内2 mg/mL泡桐花水提物对细胞内炎症因子一氧化氮(NO)的清除能力最显著。[结论]泡桐花水提物具有较显著的抑菌抗炎活性,可作为理想的饲料添加剂加以开发。[Objective]To investigate the development and utilization of Paulownia elongate flower resources in feed additives.[Method]Different extracts from P.elongate S.Y.Hu flowers were prepared.The antibacterial activities of the extracts were determined by Oxford cup method,and the minimum inhibitory concentration(MIC)of the extracts was determined by gradient dilution method.The anti-inflammatory activity of the extracts was evaluated by using xylene-induced ear swelling model and LPS induced macrophage RAW264.7 inflammatory model.[Result]The aqueous extract from P.elongate flowers had the most significant inhibitory effects on Staphylococcus aureus,Mlicrococcus tetragenus,Escherichia coli and Salmonella paratyphi,while the alcohol extract inhibited on Candida albicans.The aqueous extract from P.elongate flowers had the best anti-inflammatory effects,2 mg/mL aqueous extract from P.elongate flowers had the most significant scavenging ability on NO as intracellular inflammatory factor within the experimental concentration range.[Conclusion]The aqueous extract from P.elongate flowers had significant antibacterial and anti-inflammatory activities,so it could be used and developed as an ideal feed additive.

关 键 词：泡桐花提取物 抗菌 抗炎 

分 类 号：S816.7[农业科学—饲料科学]