SARS-CoV-2 infection causes immunodeficiency in recovered patients by downregulating CD19 expression in B cells via enhancing B-cell metabolism  

作　　者：Yukai Jing Li Luo Ying Chen Lisa S.Westerberg Peng Zhou Zhiping Xu Andres A.Herrada Chan-Sik Park Masato Kubo Heng Mei Yu Hu Pamela Pui-Wah Lee Bing Zheng Zhiwei Sui Wei Xiao Quan Gong Zhongxin Lu Chaohong Liu 

机构地区：[1]Department of Medical Laboratory,The Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,People's Republic of China [2]Department of Emergency,Shanxi Bethune Hospital,Shanxi Academy of Medical Sciences,Tongji Shanxi Hospital,Third Hospital of Shanxi Medical University,Taiyuan,People's Republic of China [3]Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,People's Republic of China [4]Department of Pathogen Biology,School of Basic Medicine,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,People's Republic of China [5]Center for Biosafety Mega Science,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan,People's Republic of China [6]Department of Microbiology Tumor and Cell Biology,Karolinska Institute,Stockholm,Sweden [7]Wuhan Metware Biotechnology Co.,Ltd,Wuhan,People's Republic of China [8]Lymphatic and Inflammation Research Laboratory,Facultad de Ciencias de la Salud,Instituto de Ciencias BiomOdicas,Universidad Autdnoma de Chile,Talca,Chile [9]Department of Pathology,Asan Medical Center,University of Ulsan College of Medicine,Seoul,Korea [10]Laboratory for Cytokine Regulation,Center for Integrative Medical Science(IMS),RIKEN Yokohama Institute,Kanagawa,Japan [11]Institute of Hematology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,People's Republic of China [12]Department of Paediatrics and Adolescent Medicine,LKS Faculty of Medicine,The University of Hong Kong,Hong Kong,People's Republic of China [13]Department of Immunology,School of Medicine,Yangtze University,Jingzhou,Hubei Province,People's Republic of China [14]Clinical Molecular Immunology Center,School of Medicine,Yangtze University,Jingzhou,Hubei Province,People's Republic of China [15]Center for Advanced Measurement Science,National Institute of Metrology,Beijing,People's Republic of China [16]Department of Respiratory,The First Affiliated Hospital of Yangtze University,Jingzhou,Hub

出　　处：《Signal Transduction and Targeted Therapy》2021年第10期3119-3131,共13页信号转导与靶向治疗（英文）

基　　金：supported by the National Natural Science Foundation of China(31970839);the National Key R&D Program of China(1316203);Independent Innovation Research Fund of Huazhong University of Science and Technology(2020kfyXGYJ017);the HUST Academic Frontier Youth Team(2018QYTD10).

摘　　要：The SARS-CoV-2 infection causes severe immune disruption.However,it is unclear if disrupted immune regulation still exists and pertains in recovered COVID-19 patients.In our study,we have characterized the immunephe no type of B cells from 15 recovered COVID-19 patients,and found that healthy controls and recovered patients had similar B-cell populations before and after BCR stimulation,but the frequencies of PBC in patients were significantly increased when compared to healthy controls before stimulation.However,the percentage of unswitched memory B cells was decreased in recovered patients but not changed in healthy controls upon BCR stimulation.Interestingly,we found that CD19 expression was significantly reduced in almost all the B-cell subsets in recovered patients.Moreover,the BCR signaling and early B-cell response were disrupted upon BCR stimulation.Mechanistically,we found that the reduced CD19 expression was caused by the dysregulation of cell metabolism.In conclusion,we found that SARS-CoV-2 infection causes immunodeficiency in recovered patients by downregulating CD19 expression in B cells via enhandng B-cell metabolism,which may provide a new intervention target to cure COVID-19.

关 键 词：PATIENTS METABOLISM CD19 

分 类 号：R373[医药卫生—病原生物学]