杀鲑气单胞菌灭活疫苗的制备及其在大菱鲆体内的免疫效果  

Preparation of Aeromonas salmonicida inactivated vaccine and test of its immunological efficacy in Scophthalmus maximus

作　　者：郭慧敏 丁圆媛 王昊 郑旭佳 修云吉 周顺[1] GUO Huimin;DING Yuanyuan;WANG Hao;ZHENG Xujia;XIU Yunji;ZHOU Shun(School of Marine Science and Engineering,Qingdao Agricultural University,Qingdao266200,China)

机构地区：[1]青岛农业大学海洋科学与工程学院,山东青岛266200

出　　处：《水产学报》2021年第9期1574-1583,共10页Journal of Fisheries of China

基　　金：山东省现代农业产业技术体系鱼类产业创新团队(SDAIT-12-06);山东省2018年度农业重大应用技术创新项目。

摘　　要：为了预防大菱鲆疥疮病,本研究将杀鲑气单胞菌菌株HHSM1905经甲醛灭活制备成灭活疫苗,通过腹腔注射途径免疫健康大菱鲆;对免疫后大菱鲆血清抗体效价、血清溶菌酶(LZM)活性、酸性磷酸酶(ACP)活性及疫苗保护率进行测定;以β-actin为内参,对免疫相关基因(IL-1β、TLR-5、MHCⅠ、MHCⅡ-α、CD4)表达情况进行定量PCR分析。结果显示,在免疫后2周时,疫苗组血清抗体效价显著高于对照组并达到最大值;疫苗组LZM活性在免疫2周时达到峰值,4周时仍与对照组保持显著差异;疫苗组ACP活性在免疫后的2周达到峰值,与对照组差异显著;灭活疫苗相对保护率(RPS)为72.72%。免疫组与对照组相比,IL-1β、TLR-5、MHCⅠ、MHCⅡ-α、CD4的表达量均呈上调趋势,与对照组差异显著。本研究成功制备了大菱鲆用杀鲑气单胞菌灭活疫苗,其对大菱鲆疥疮病能够起到一定的预防作用,可为大菱鲆养殖过程中疾病预防提供新方法。Scophthalmus maximus is one of the important species of mariculture in China, and occupies an important position in China’s mariculture industry. At present, S. maximus farming industry shows a rapid growth trend,but as S. maximus farming intensification and scale continue to increase, bacterial diseases continue to occur. As pathogens of scabies, Aeromonas salmonicida has caused great economic losses to aquaculture. In order to prepare the inactivated vaccine of A. salmonicida and evaluate its immune effect on S. maximus, HHSM1905 strains were killed by formalin to prepare the inactivated vaccine. The experimental animals were randomly divided into two groups, i.e. immune group and control group. S. maximus was immunized by intraperitoneal injection. The indexes such as serum antibody titer, serum lysozyme activity, acid phosphatase activity and vaccine protection rate were determined. The expression of related immune genes(IL-1β, TLR-5, MHC Ⅰ, MHC Ⅱ-α and CD4) was studied with β-actin gene as internal reference. The results showed that 2 weeks after vaccination, the antibody titer of immune group was significantly higher than that of control group and reaching the peak value. The lysozyme activity of the immune group reached the peak value 2 weeks after immunization, and remained significantly different from that of the control group at 4 weeks;the acid phosphatase activity of the immune group reached the peak value at 2 weeks after immunization;the relative percentage survival of the combined vaccine was 72.72%.Compared with the control group, IL-1β, TLR-5, MHC Ⅰ, MHC Ⅱ-α and CD4 immune genes in the immune group had an upward trend, and most of them reached the maximum value at 1-2 weeks, which had significant difference with the control group. In conclusion, the inactivated vaccines of A. salmonicida for S. maximus were successfully prepared in this study. The A. salmonicida inactivated vaccine was effective for preventing A. salmonicida disease,and may provide a novel method for disease preven

关 键 词：大菱鲆 杀鲑气单胞菌 疥疮病 灭活疫苗 

分 类 号：S942.5[农业科学—水产养殖]