过氧乙酸/乙醇对异体皮性能的影响  

Effect of peracetic acid/ethanol on the properties of allogeneic skin

作　　者：胡凯 陈维明 骆井万 李淼 汪晶晶 李矛 沈亚俊 陈金发 白玉龙 Hu Kai;Chen Weiming;Luo Jingwan;Li Miao;Wang Jingjing;Li Mao;Shen Yajun;Chen Jinfa;Bai Yulong(Changzhou Gencong Biomechanics Research Center,Changzhou 213003,Jiangsu Province,China;Shanghai Yapeng Biotechnology Co.,Ltd.,Shanghai 201201,China;Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China;Beijing Dental Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]常州市亘从生物力学研究中心,江苏省常州市213003 [2]上海亚朋生物技术有限公司,上海市201201 [3]首都医科大学附属北京同仁医院,北京市100730 [4]首都医科大学附属北京口腔医院,北京市100050

出　　处：《中国组织工程研究》2022年第10期1537-1543,共7页Chinese Journal of Tissue Engineering Research

基　　金：2020年上海市科技型中小企业技术创新资金(200H1029400),项目负责人:陈维明。

摘　　要：背景:过氧乙酸/乙醇是一种理想的动物源及同种异体组织病毒灭活试剂,其对病原微生物杀灭效果显著,但是未见其对异体皮其他性能影响的研究报道。目的:研究过氧乙酸/乙醇处理对异体皮性能的影响。方法:配制3种不同浓度的过氧乙酸/乙醇溶液,2.3 g/L过氧乙酸+体积分数24%乙醇(设为P/E-1)、1.8 g/L过氧乙酸+体积分数4.8%乙醇(设为P/E-2)、15.33 g/L过氧乙酸+体积分数24%乙醇(设为P/E-3)。采用不同浓度的过氧乙酸/乙醇溶液分别处理异体皮15,30,45,60 min,通过外观、组织学染色、力学性能、糖胺聚糖含量检测等评价皮肤结构性能变化,并检测经处理后皮肤的化学试剂残留情况和细胞毒性。结果与结论:①各浓度过氧乙酸/乙醇溶液处理不同时间的异体皮外观无明显差异;②苏木精-伊红染色显示,与未处理的异体皮相比,P/E-1与P/E-2处理15 min后的皮肤细胞外基质结构无明显变化,处理15 min以上的皮肤细胞外基质结构相对疏松,但保留了结构的完整性;P/E-3处理15 min时,皮肤真皮层中出现大孔,处理15 min以上后真皮层出现较多孔洞,细胞外基质结构被严重破坏;③同一浓度溶液处理下,处理时间越长,皮肤的糖胺聚糖含量、拉伸强度和弹性模量越低;相同处理时间下,P/E-1处理组拉伸强度最大、P/E-3处理组最小,P/E-2处理组断裂伸长率最大、P/E-3处理组最小,P/E-3处理组韧性最小;④试剂残留检测显示,过氧乙酸和乙醇可从异体皮去除,残留量在规定的限度要求内,无明显的细胞毒性;⑤结果显示在相同的处理时间下,P/E-1组皮肤的性能及结构与原材料最接近。BACKGROUND:The peracetic acid/ethanol is an ideal reagent for inactivating viruses of animal derived tissues and allogeneic tissues.It is effective in killing pathogenic microorganisms.However,its effect on other properties of allogeneic skin has not been reported.OBJECTIVE:To investigate the effect of peracetic acid/ethanol treatment on the performance of allogeneic skins.METHODS:Three different concentrations of peracetic acid/ethanol solutions were prepared,which were 2.3 g/L peracetic acid+24%v/v ethanol(set as P/E-1),1.8 g/L peracetic acid+4.8%v/v ethanol(set as P/E-2)and 15.33 g/L peroxyacetic acid+24%v/v ethanol(set as P/E-3).Allogeneic skin was treated with different concentrations of peracetic acid/ethanol for 15,30,45 and 60 minutes,respectively.The structural properties of the skin were evaluated via appearance,histological staining,mechanical test and glycosaminoglycan content test.Besides,chemical reagent residues and cytotoxicity in the treated skin were also studied.RESULTS AND CONCLUSION:(1)No significant difference in the epidermal appearance of skin were observed at all investigated concentrations of peracetic acid/ethanol.(2)Hematoxylin-eosin staining showed no obvious changes in the extracellular matrix structure of skin after 15 minutes of P/E-1 and P/E-2 treatment compared with untreated allogeneic skin,and the extracellular matrix structure of skin treated with more than 15 minutes was relatively loose which preserved an integral structure.However,microporous appeared in the dermis while it was treated for 15 minutes,especially after 15 minutes,more holes appeared in the dermis and the extracellular matrix structure was seriously destroyed.(3)The longer the treatment time at the same concentration,the lower the glycosaminoglycan content and the lower the mechanical strength and modulus.With the same treatment time,the tensile strength of the P/E-1 treatment group was the largest,while the P/E-3 treatment group was the smallest;the elongation at break of the P/E-2 treatment group was the lar

关 键 词：材料 病毒灭活 异体皮 脱细胞真皮基质 过氧乙酸/乙醇法 

分 类 号：R459.9[医药卫生—治疗学] R392.4[医药卫生—临床医学]