转化生长因子β3复合海藻酸钠水凝胶修复膝关节软骨缺损  

Transforming growth factor beta 3 and alginate hydrogel complex on the repair of articular cartilage defects of the knee

作　　者：刘莹松 郭晓鹏 魏明珠 Liu Yingsong;Guo Xiaopeng;Wei Mingzhu(Department of Orthopedics,Taihe Hospital(Affiliated Hospital of Hubei University of Medicine),Shiyan 442000,Hubei Province,China;Functional Department of Integrated Traditional Chinese and Western Medicine Hospital,Shiyan 442000,Hubei Province,China)

机构地区：[1]十堰市太和医院骨科(湖北医药学院附属医院),湖北省十堰市442000 [2]十堰市中西医结合医院功能科,湖北省十堰市442000

出　　处：《中国组织工程研究》2022年第16期2504-2509,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:已经有大量研究报道转化生长因子β3与藻酸盐、间充质干细胞复合修复软骨缺损的动物实验,但是关于转化生长因子β3与海藻酸钠水凝胶复合间充质干细胞修复软骨损伤的研究较少。目的:对比海藻酸钠水凝胶与负载转化生长因子β3的海藻酸钠水凝胶分别复合骨髓间充质干细胞修复骨缺损的效果。方法:体外分离培养兔骨髓间充质干细胞备用;制备转化生长因子β3海藻酸钠水凝胶复合物;移植前,将骨髓间充质干细胞悬液与水凝胶等体积混合。取48只新西兰大白兔制备膝关节软骨缺损,随机分3组:损伤组不作任何处理,对照组植入海藻酸钠水凝胶与骨髓间充质干细胞悬液,观察组植入转化生长因子β3海藻酸钠水凝胶复合物与骨髓间充质干细胞悬液。术后12周时取材,分别进行大体、组织学观察、Ⅱ型胶原免疫组化染色与RT-PCR检测。结果与结论:(1)大体观察:术后12周时,损伤组缺损部位由大量肉芽组织填充,对照组由半透明状软骨样组织填充,观察组由新生组织填满;(2)组织学观察:术后12周苏木精-伊红染色与番红O染色可见,损伤组缺损部位被较多的纤维组织填充,中央部位存在较大的缝隙;对照组可见较多的软骨样组织与成纤维组织,表面欠规则,材料大部分降解,材料周围由大量的骨小梁包绕;观察组可见大量的软骨样组织,表面较光滑且结构较致密,类似于周围正常软骨组织;(3)Ⅱ型胶原免疫组化染色:损伤组仅见极其微弱的阳性染色,对照组、观察组可见阳性染色,并且观察组的阳性着色程度明显强于对照组;(4)RT-PCR检测:对照组再生软骨组织的Ⅱ型胶原、Sox9、糖胺多糖mRNA表达均低于观察组(P<0.05);(5)结果表明:负载转化生长因子β3的海藻酸钠水凝胶-骨髓间充质干细胞复合物可促进关节软骨缺损的修复,提升关节软骨基因的表达。BACKGROUND: Numerous studies have reported that transforming growth factor beta 3 combined with alginate and mesenchymal stem cells can repair cartilage defects in animal experiments. However, there is little research about the effect of transforming growth factor beta 3 and sodium alginate hydrogel combined with mesenchymal stem cells on repairing cartilage injury. OBJECTIVE: To compare effects of sodium alginate hydrogel and sodium alginate hydrogel loaded with transforming growth factor beta 3 on bone defect with bone marrow mesenchymal stem cells.METHODS: Rabbit bone marrow mesenchymal stem cells were isolated and cultured in vitro. A suitable amount of transforming growth factor beta 3 was added into sodium alginate solution. Before transplantation, bone marrow mesenchymal stem cell suspension was mixed with the hydrogel in equal volumes. Totally 48 New Zealand white rabbits were used to prepare unilateral articular cartilage defects and randomly divided into three groups. The injury group did not take any treatment;the control group was implanted with alginate hydrogel and bone marrow mesenchymal stem cell suspension;and the observation group was implanted with transforming growth factor beta 3-sodium alginate hydrogel complex and bone marrow mesenchymal stem cell suspension. At 12 weeks after operation, the samples were taken for gross and histological observation and type II collagen immunohistochemical staining and RT-PCR. RESULTS AND CONCLUSION:(1) General observation: At 12 weeks, the defect site of the injury group was filled with a large number of granulation tissue;the control group was filled with translucent cartilage like tissue;and the observation group was filled with new tissue.(2) Histological observation: At 12 weeks after operation, hematoxylin-eosin staining and safranin O staining showed that the defect site in the injury group was filled with more fibrous tissue, and there was a large gap in the central part. In the control group, there were more cartilage like tissue and fibroblast tiss

关 键 词：软骨 材料 水凝胶 转化生长因子Β3 海藻酸钠水凝胶 软骨缺损 软骨组织工程 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]