黄晶果采后主要病原菌的分离与鉴定  

Isolation and Identification of Dominant Pathogens of Postharvest Abiu Fruit

作　　者：徐丹丹 乔方[1] 刘敏 林泽勉 禤文生 XU Dan-dan;QIAO Fang;LIU Min;LIN Ze-mian;XUAN Wen-sheng(School of Applied Chemistry and Biotechnology,Postdoctoral Innovation Practice Base,Shenzhen Polytechnic,Shenzhen 518055,China;Shenzhen Agricultural Product Quality and Safety Inspection and Testing Center,Shenzhen 518005,China;College of Plant Protection,Guangdong Province Key Laboratory of MicrobialSignals and Disease Control,South China Agricultural University,Guangzhou 510642,China;Guangdong Longfree Garden,Jiangmen 529200,China)

机构地区：[1]深圳职业技术学院应用化学与生物技术学院,深圳职业技术学院博士后创新实践基地,广东深圳518055 [2]深圳市农产品质量安全检验检测中心,广东深圳518005 [3]华南农业大学植物保护学院,广东省微生物信号与作物病害防控重点实验室,广东广州510642 [4]广东龙飞生物有限公司,广东江门529200

出　　处：《保鲜与加工》2021年第11期94-101,共8页Storage and Process

基　　金：深圳职业技术学院博士后启动基金项目(6019330008K)。

摘　　要：为明确引起黄晶果采后腐烂的主要致病菌,采用传统组织分离法从采后贮藏期自然发病的黄晶果果实上分离病原菌,利用柯赫氏法则进行致病性测定;结合形态学特征和联合基因(ITS和TUB2)系统进化树分析进行病原菌鉴定。结果表明:从黄晶果果实上分离得到的5株疑似病原菌,回接发病症状与自然贮藏发病症状一致,确定其为黄晶果的病原菌;5株病原菌分别为:可可毛色二孢(Lasiodiplodia theobromae)、蔷薇拟盘多毛孢(Neopestalotiopsis rosae)、间座壳真菌(Diaporthe pseudomangiferae)、层出镰孢菌(Fusarium proliferatum)和棘孢曲霉(Aspergillus aculeatinus)。该研究通过对黄晶果采后贮藏期间病原菌的分离鉴定,为黄晶果采后病害的有效防控及延长其采后贮藏时间提供科学依据。In order to clarify the species of pathogenic microorganisms causing postharvest decay of abiu fruit,the pathogens were isolated from naturally infected abiu during the postharvest storage using traditional tissue isolation method,and Koch’s postulates was used to determine their pathogenicity.The pathogen identification were carried out according to the morphological characterization and phylogenetic tree(ITS and TUB2)analysis.The results showed that five obtained isolates caused similar disease symptoms when re-inoculated to healthy abiu,which indicated their pathogenicity.Five pathogens were identified as Lasiodiplodia theobromae,Neopestalotiopsis rosae,Diaporthe pseudomangiferae,Fusarium proliferatum and Aspergillus aculeatinus.The purpose of the study is to provide a scientific basis for effecive prevention and control of postharvest disease of abiu fruit and extension of the storage time.

关 键 词：黄晶果 采后病原菌 果实腐烂 分离鉴定 

分 类 号：S662.9[农业科学—果树学]