固定化Alcaligenes faecalis WT14对累积亚硝酸盐的还原  

Reduction of Accumulated Nitrite by Immobilized Alcaligenes FaecalisWT14 during Aerobic Denitrification Process

作　　者：陈均利 刘锋[1,2] 张树楠 肖润林[1] 何铁光[4] 吴金水 CHEN Junli;LIU Feng;ZHANG Shunan;XIAO Runlin;HE Tieguang;WU Jinshui(Key Laboratory of Agro-ecological Processes in Subtropical Regions,Changsha Research Station for Agricultural and Environmental Monitoring,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410125,China;University of Chinese Academy of Sciences,Beijing 100049,China;Hunan Jiahe Breeding Intelligence Service Co.,Ltd,Changsha 410199,China;Agricultural Resources and Environment Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China)

机构地区：[1]中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验,长沙农业环境观测研究站,湖南长沙410125 [2]中国科学院大学,北京100049 [3]湖南佳和智农技术咨询有限公司,湖南长沙410199 [4]广西壮族自治区农业科学院农业资源与环境研究所,广西南宁530007

出　　处：《水处理技术》2021年第11期65-70,共6页Technology of Water Treatment

基　　金：长江科学院开放研究基金资助项目(CKWV2021890/KY);中国科学院战略性先导科技专项(XDA23020402,XDA23020502);湖南省高新技术产业科技创新引领计划项目(2020SK2034、2020NK2004);湖南省2020年环保科研项目。

摘　　要：针对好氧反硝化菌在好氧条件有着良好的硝酸盐去除能力,但亚硝酸盐的积累阻碍了TN的去除的问题,采用聚乙烯醇混合海藻酸钠、活性炭粉末和氯化钙的方式对好氧反硝化菌WT14进行固定化,并应用固定化菌去除实际水产养殖废水中的硝酸盐。结果表明,克服了亚硝酸盐积累问题,显著提高了TN去除率。与游离菌相比,固定化菌有着更高的TN去除率(分别为30.2%和55.5%),优化脱氮条件为,20℃、pH 9.0和60 r/min。固定化菌循环使用8个周期均有着稳定的TN去除率(92.6%)。用以处理实际水产养殖废水时,硝酸盐去除率为100%,且不含亚硝酸盐的积累。说明好氧反硝化联合固定化技术在实际应用中具有良好潜力。Aimed at aerobic denitrifying bacteria has good nitrate removal ability in aerobic conditions,but the accumulation of nitrite hinders the removal of TN.The polyvinyl alcohol was mixed with sodium alginate,activated carbon powder,and calcium chloride to immobilize aerobic denitrifying bacteria WT14,and used immobilized bacteria to remove nitrate in actual aquaculture wastewater.The results showed that,the problem of nitrite accumulation was overcome,and the TN removal rate was significantly improved.Compared with free bacteria,immobilized bacteria had a higher TN removal rate(30.2%and 55.5%,respectively),and the optimal nitrogen removal conditions were 20℃,pH of 9.0 and 60 r/min.The immobilized bacteria had a stable TN removal rate(92.6%)for 8 cycles.The treatment of actual aquaculture wastewater also showed the nitrate removal rate was 100%,and did not contain nitrite accumulation.These results indicated that the aerobic denitrification combined immobilization technology has good potential in practical applications.

关 键 词：好氧反硝化 Alcaligenes faecalis WT14 硝酸盐 亚硝酸盐 固定化技术 

分 类 号：X714[环境科学与工程—环境工程]