桑寄生总黄酮对链脲佐菌素诱导糖尿病小鼠降糖作用机制研究  

Hypoglycemic Effect of Total Flavonoids from Mistletoe on Diabetic Mice Induced by Streptozotocin

作　　者：蒙田秀[1] 袁小玲 梁芳[1] 杨立芳 龚志强[1,2] MENG Tianxiu;YUAN Xiaoling;LIANG Fang;YANG Lifang;GONG Zhiqiang(Saiensin School ofMedicine,Guangxi University of Traditional Chinese Medicine,Nanning 530222,China;School of Chemistry and Chemical Engineering,Guangxi University for Nationalities,Nanning 530003,China)

机构地区：[1]广西中医药大学赛恩斯新医药学院,广西南宁530222 [2]广西民族大学化学化工学院,广西南宁530003

出　　处：《陕西中医药大学学报》2021年第6期55-60,共6页Journal of Shaanxi University of Chinese Medicine

基　　金：广西自然科学基金资助(2018GXNSFBA281033);广西高校中青年教师基础能力提升项目(2018KY0862);2018年国家级大学生创新创业项目(201813643017)。

摘　　要：目的以桑寄生总黄酮(TFHT)为研究对象,探讨其治疗糖尿病的效果及其作用机制。方法采用大孔树脂富集桑寄生中总黄酮,紫外分光光度法测量总黄酮含量,通过考察TFHT对α-葡萄糖苷酶和α-淀粉酶活性的抑制,研究TFHT体外降糖作用。用TFHT的不同剂量连续4周治疗链脲佐菌素(STZ)诱导的糖尿病小鼠,每周测1次血糖,最后1周末取血测定相关指标,取血后处死取肝脏检测超氧化物歧化酶(SOD)、丙二醛(MDA)及肝糖原的含量,来研究TFHT体内降糖效果。结果体外降血糖实验中TFHT对α-葡萄糖苷酶活性的抑制作用明显,且剂量加大抑制作用增强,但对α-淀粉酶活性的抑制作用不显著。体内降血糖结果显示,与模型组比较,TFHT不同剂量降血糖效果明显有统计学差异,随着其葡萄糖耐受能力的增强,血清胰岛素的含量也得到明显提高。同时,TFHT给药后糖尿病小鼠肝糖原含量增加明显,SOD活性升高,MDA含量降低,胸腺指数升高。结论桑寄生总黄酮有良好的降糖作用,其作用机制可能通过抑制小鼠体内脂质过氧化,保护糖尿病小鼠胸腺萎缩及改善胰岛素抵抗有关。Objective To study the effect and mechanism of total flavonoids(TFHT)from mistletoe on diabetes mellitus.Methods Macroporous resin was used to enrich the total flavonoids from mistletoe,and the total flavonoid content was measured by UV spectrophotometry.The TFHT pair was measured by the method.The inhibition of α-Glucosidase andα-amylase activity was used to study the hypoglycemic effect of TFHT.4 consecutive weeks of treatment with different doses of TFHT were used to treat diabetic mice induced by streptozotocin(STZ)for 1 times a week.Blood glucose was measured at the end of 1 weeks,and blood samples were taken to detect superoxide dismutase(SOD),malondialdehyde(MDA)and liver glycogen to study the hypoglycemic effect of TFHT in vivo.Results In vitro hypoglycemic experiment,TFHT has obvious inhibitory effect onα-glucosidase activity,and the inhibitory effect was enhanced with the increase of dose,while it has no significant inhibition ofα-amylase activity.In vivo hypoglycemic results show that,compared with the model group,the hypoglycemic effect of different doses of TFHT was significantly different.With the enhancement of glucose tolerance,the content of serum insulin was also significantly increased.Meanwhile,after TFHT administration,the liver glycogen content of diabetic mice increased significantly,SOD activity increased,MDA content decreased,and thymus index increased.Conclusion The total flavonoids of mistletoe has good hypoglycemic effect,and its mechanism may be related to inhibiting lipid peroxidation in mice,protecting thymus atrophy and improving insulin resistance in diabetic mice.

关 键 词：桑寄生 总黄酮 降血糖 链脲佐菌素 作用机制 

分 类 号：R285[医药卫生—中药学]