男男性行为者急慢性HIV感染者口腔菌群结构及多样性分析  

Comparative analysis of the structure and diversity of oral microbiomes in men who have sex with men with acute and chronic HIV infections

作　　者：李双 粟斌 朱俊萍[2] 何秋水[2] 张彤 LI Shuang;SU Bin;ZHU Junping;HE Qiushui;ZHANG Tong(Beijing Key Laboratory for HIV/AIDS Research,Center for Infectious Diseases,Beijing Youan Hospital,Capital Medical University,Beijing 100069,China;Department of Medical Microbiology and Research Centre of Microbiome,Capital Medical University,Beijing 100069)

机构地区：[1]首都医科大学附属北京佑安医院感染中心,艾滋病研究北京市重点实验室,北京100069 [2]首都医科大学基础医学院病原生物学系,北京100069

出　　处：《中国艾滋病性病》2021年第10期1075-1079,共5页Chinese Journal of Aids & STD

基　　金：国家“十三五”科技重大专项(2017ZX10202101-004-001,2017ZX10202102-005-003);国家自然科学基金项目(81772165,81974303,82072271);艾滋病研究北京市重点实验室(BZ0089)。

摘　　要：目的探讨MSM急、慢性HIV感染者与MSM中HIV未感染者口腔菌群的变化规律和差异。方法2019年5-11月,收集15例MSM急性HIV感染者、15例MSM慢性HIV感染者以及15例未感染HIV的MSM健康对照者的咽拭子标本。提取咽拭子基因组DNA进行16S rRNA基因PCR扩增,然后对其V4~V5区域进行测序,并对口腔菌群群落结构及多样性进行差异分析。结果在门水平上,以厚壁菌门、变形菌门、拟杆菌门和梭杆菌门为主,这四大菌门占据全部菌群成员的90%以上,其中对照组中四大菌门所占比例为91.92%,急性HIV感染组为97.73%,慢性HIV感染组为98.62%。与MSM对照组相比,MSM急、慢性HIV感染组口腔菌群丰度和多样性均显著下降,并且群落结构组成存在显著差异。在属水平上,MSM急、慢性HIV感染组的丁酸弧菌属、拟杆菌属、劳特罗普菌属、乳杆菌属、罗氏菌属、棒状杆菌属等丰度显著低于MSM对照组;MSM急性HIV感染组金氏菌属、普氏菌属丰度显著高于MSM对照组,而纤毛菌属显著低于MSM对照组;MSM慢性HIV感染组链球菌属丰度显著高于MSM对照组,而放线菌属丰度显著低于MSM对照组;MSM慢性HIV感染组的假单胞菌属、金氏菌属和Oribacterium丰度与MSM急性HIV感染组相比显著降低。结论MSM急、慢性期HIV感染者口腔菌群丰度和多样性与健康对照组相比存在显著差异;急、慢性HIV感染组之间的口腔菌群组成及丰度也存在差异。Objective To explore the changes and differences of oral microbiomes among MSM with acute and chronic HIV infections and MSM without HIV infection.Methods From May to November 2019,throat swabs were collected from 15 patients with acute HIV infection,15 patients with chronic HIV infection(both were from MSM population)and 15 MSM without HIV infection.The genomic DNAs were extracted from swabs and amplified by PCR for the sequencing of 16 S rRNA V4-V5 region.The differences in structure and diversity of oral microbiomes were analyzed.Results At the phylum level,the four phyla were mainly Firmicutes,Proteobacteria,Bacteroidetes,Fusobacterium which accounted for more than 90%of all bacteria members.The proportion of the four phyla in the MSM without HIV infection group was 91.92%,that in the acute HIV infection group was 97.73%and that in the chronic HIV infection group was 98.62%.Compared with the MSM without infection,the abundance and diversity of oral microbiomes in both acute and chronic HIV infection groups were decreased significantly,and there were significant differences in community structure.At the genus level,the abundance of Butyrivibio,Bacteroides,Lautropia,Lactobacillus,Rothia and Corynebacterium in the acute and chronic HIV infection groups were significantly lower than those in the MSM without infection group.The abundance of Kingella and Prevotella in the acute HIV infection group were significantly higher than that in MSM without infection group,while the Leptotrichia was significantly lower than that in MSM without infection group.The abundance of Streptococcus in chronic HIV infection group was significantly higher than that in MSM without infection group,while the abundance of Actinomyces was significantly lower than that in MSM without infection group.The abundance of Pseudomonas,Kingella and Oribacterium in chronic HIV infection group were significantly lower than that in acute HIV infection gourp.Conclusion The abundance and diversity of oral microbiomes in acute and chronic HIV infection pa

关 键 词：艾滋病病毒 口腔菌群 16SrRNA 菌群多样性 

分 类 号：R373.9[医药卫生—病原生物学]