梅毒螺旋体核酸扩增检测技术及基因分型方法研究进展  

Review on the nucleic acid amplification test and genotyping method for Treponema pallidum

作　　者：张瑾 陈绍椿[1] 尹跃平[1] ZHANG Jin;CHEN Shaochun;YIN Yueping(Institute of Dermatology,Chinese Academy of Medical Sciences and Peking Union Medical College,National Center for Sexually Transmitted Disease Control,China Center for Disease Control and Prevention,Nanjing 210042,China)

机构地区：[1]中国医学科学院,北京协和医学院皮肤病研究所,国家性病控制中心,中国疾病预防控制中心,南京210042

出　　处：《中国艾滋病性病》2021年第10期1174-1178,共5页Chinese Journal of Aids & STD

基　　金：“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项(2018ZX10101001-004-003);江苏省高层次卫生人才“六个一工程”拔尖人才项目(LGY2019091)。

摘　　要：梅毒是主要的性传播疾病之一,其临床诊断依赖于实验室检测结果。梅毒的实验室检测技术主要包括病原学检测和血清学检测,血清学检测是临床诊断常用方法,近年来病原学检测也有所发展,特别是核酸扩增检测技术发展较快,基于核酸扩增的基因分型方法也广泛应用于梅毒分子流行病学研究。为更好了解并正确使用梅毒核酸扩增及基因分型技术,本文将对这两项技术近年来的研究进展进行综述和讨论。Syphilis is one of the most common sexually transmitted diseases.The diagnosis of syphilis depends on laboratory tests,which mainly include etiological testing for its pathogen Treponema pallidum and serological testing for antibodies.Serological testing is commonly used for clinical diagnosis.In recent years,the nucleic acid amplification test(NAAT),is a kind of etiological testing,has being developed rapidly,and molecular genotyping based on NAAT has been widely used in the molecular epidemiology research of syphilis.To better understand and proper use of the NAAT and genotyping methods for syphilis,we reviewed the latest progress and discussed the application of these two techniques.

关 键 词：梅毒螺旋体 核酸扩增检测 基因分型 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]