基于胶原-琼脂糖的组织工程表皮替代物构建研究  

Research on the Construction of Tissue Engineering Epidermal Substitute Based on Collagen-Agarose

作　　者：赵雅静 曹燕燕 申治中[1,2] 菅傲群 桑胜波 ZHAO Yajing;CAO Yanyan;SHEN Zhizhong;JIAN Aoqun;SANG Shengbo(Micro-Nano System Research Center,College of Information and Computer,Taiyuan University of Technology,Taiyuan 030024,China;Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education,Taiyuan University of Technology,Taiyuan 030024,China;College of Information Science and Engineering,Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]太原理工大学信息与计算机学院微纳系统研究中心,太原030024 [2]太原理工大学新型传感器与智能控制教育部和山西省重点实验室,太原030024 [3]河北北方学院信息科学与工程学院,河北张家口075000

出　　处：《太原理工大学学报》2021年第6期1003-1009,共7页Journal of Taiyuan University of Technology

基　　金：国家自然科学基金资助项目(51975400,61703298);国家重点研究发展项目资助(2019YFB1310200);山西省优秀人才创新科技计划(201805D211020)。

摘　　要：从开发新的组织工程表皮替代物出发,选择胶原蛋白和琼脂糖构建复合水凝胶,用于支持表皮细胞生长。采用质量分数2%的胶原蛋白溶液和质量分数2%的琼脂糖溶液构建复合水凝胶,以胶原凝胶作为对照,分别测试胶原-琼脂糖复合水凝胶的形貌、理化性能和机械性能;观察胶原-琼脂糖复合水凝胶与人类永生化表皮(HaCaT)细胞的生物相容性。研究表明,琼脂糖可以改善胶原蛋白的机械性能,当胶原-琼脂糖复合水凝胶支架中两者的含量分别为65%和35%时,可以同时兼顾支架的机械性能与生物相容性。基于胶原-琼脂糖的复合水凝胶是一种理想的组织工程表皮替代物。From the perspective of developing new tissue engineering epidermal substitutes,collagen and agarose were selected to construct a composite hydrogel to support the growth of epidermal cells.Collagen solution(2%)and agarose solution(2%)were used to investigate the morphology,physical and chemical properties and mechanical properties of the collagen-agarose composite hydrogels with collagen as a control.The biocompatibility of collagen-agarose composite hydrogel with human immortalized epidermal cells(HaCaT)was also examined.The results show that agarose improved the mechanical properties of collagen.The mechanical properties and biocompatibility of the scaffold were balanced when the content of collagen and agarose is 65%and 35%,respectively.The composite hydrogel based on collagen-agarose developed in this study is an ideal epidermal substitute for tissue engineering.

关 键 词：胶原 琼脂糖 人类永生化表皮细胞 表皮替代物 组织工程 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]