福建省1株Salivirus病毒全基因组特征分析  

Genome-wide characterization of a salivirus strain isolated in Fujian province

作　　者：何文祥[1] 吴冰珊[1] 林琦[1] 陈炜[1] 翁育伟[1] HE Weng-xiang;WU Bing-shan;LIN Qi(Fujian Provincial Key Laboratory of Zoonosis Research,Fujian Provincial Center for Disease Control and Prevention,Fuzhou,Fujian Province 350001,China)

机构地区：[1]福建省疾病预防控制中心福建省人兽共患病研究重点实验室,福州350001

出　　处：《中国公共卫生》2021年第10期1535-1539,共5页Chinese Journal of Public Health

基　　金：福建省卫生健康科技计划项目(2019–1–18)。

摘　　要：目的了解福建地区Salivirus病毒的全基因组特征并对其遗传背景进行分析。方法对2018年福建省342份腹泻儿童粪便标本中检测到的1份Salivirus病毒阳性标本进行高通量测序,获得病毒全基因组序列FUAN-01。对FUAN-01全基因组核苷酸序列和VP1区的核苷酸及氨基酸进行遗传进化分析。结果 FUAN-01全长7907 bp。全基因组与参考株的核苷酸同源性为81.86%~98.21%,与KT182636株的同源性最高,二者ORF区(开放阅读框)编码蛋白存在14个氨基酸位点的差异。VP1区与参考株的核苷酸酸同源性为76.83%~97.81%,与KT182636株的同源性最高;氨基酸同源性为85.27%~99.22%,与KT182636株同源性最高。FUAN-01属于Salivirus A1(SAV-A1)基因型。结论首次在福建地区腹泻儿童粪便中发现Salivirus。国内SAV-A1株或由同一来源传入,与欧洲来源的病毒可能存在流行病学关联。需建立可靠的筛查办法,进一步对福建地区Salivirus的病原学及流行病学情况展开研究。Objective To analyze whole genome characteristics and genetic background of a salivirus strain isolated in Fujian province. Methods Next-generation sequencing was performed on a salivirus-positive specimen detected from 342 feces samples from the children with diarrhea in Fujian province in 2018 to obtain the whole genome sequence of the virus strain named FUAN-01. The FUAN-01’ s genetic evolution in nucleotide sequence and the nucleotide and amino acid of VP1 region were analyzed. Results The full-length nucleotide of FUAN-01 is 7907 bp. The nucleotide homology between the whole genome of the virus strain and that of the reference strain is 81.86% – 98.21%, and the homology is the highest with KT182636 strain. There are differences in 14 amino acid sites of the open reading frame(ORF) region coding protein between the two strains. The nucleotide acid homology between VP1 of FUAN-01 and the reference strain was 76.83% –97.81%, and the homology is the highest with KT182636 strain. The homology of amino acids between the FUAN-01 and that of reference strain is 85.27% – 99.22% and the homology is also the highest with that of KT182636 strains. The FUAN-01 belongs to salivirus A1(SAV-A1) genotype. Conclusion A salivirus strain was first isolated in feces specimens from the children with diarrhea in Fujian province. The domestic SAV-A1 strain may be introduced from the same source and may be epidemiologically associated with the viruses of European origin. A reliable screening strategy should be established to further study the etiology and epidemiology of salivirus infection in Fujian.

关 键 词：Salivirus 全基因组 遗传进化分析 

分 类 号：R181.81[医药卫生—流行病学]