基于射频能量的肠道组织焊接研究  

Intestinal Tissue Fusion Based on Radiofrequency Energy

作　　者：胡钟欣 宗乃馨 宋成利[1] 周宇[1] 涂良勇 毛琳[1] HU Zhongxin;ZONG Naixin;SONG Chengli;ZHOU Yu;TU Liangyong;MAO Lin(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093

出　　处：《医用生物力学》2021年第5期790-796,共7页Journal of Medical Biomechanics

基　　金：国家自然科学基金项目(51735003,51901137)。

摘　　要：目的在射频能量的作用下,通过新型压力可控电极对猪小肠(回肠部分)进行焊接,验证能量组织焊接技术对于肠道结构重建的可行性和安全性。方法将新鲜猪小肠按"黏膜-浆膜"嵌套在负电极上,通过施压圆锥体对正电极施加不同的压合压强(497、796、995、1194、1492 k Pa),在射频能量作用下完成肠道组织的焊接,通过撕脱力和爆破压测试研究焊接吻合口的生物力学特性,并对组织热扩散和微观组织结构进行检查。结果在能量输出功率160 W,压合压强995 k Pa,焊接时间为13 s时,肠道吻合口呈现最优的生物力学特性,其撕脱力和爆破压分别达到(8.73±1.11)N和(8.29±0.41)k Pa,且组织微观结构较完整,并能观察到少量游离胶原蛋白。结论射频能量组织焊接技术具有良好的应用前景,能够实现肠道组织快速、稳定的连接,对缩短手术时间、简化操作流程并提高手术质量,具有重要意义。Objective The ileum of porcine intestines with radiofrequency(RF)energy was fused through a novel linkage-type pressure controlled electrode,so as to verify feasibility and security of intestinal reconstruction in the RF energy tissue fusion technology.Methods Fresh porcine intestines were fixed on negative electrode in the order of’mucosa-serosa’,and then different compressive pressures(497,796,995,1194,1492 k Pa)and RF energy were applied to the tissues through positive electrode of pressure cone to complete intestinal anastomosis.Biomechanical properties of the fused area were studied by tensile strength and bursting pressure test,and the thermal diffusion and tissue microstructure also studied.Results The anastomotic tensile strength and bursting pressure could reach(8.73±1.11)N and(8.29±0.41)kPa,respectively,when the energy output power,pressure and welding time were 160 W,995 k Pa and 13 s,respectively,and an intact microstructure with little free collagen in the fused area could be observed.Conclusions The technology of RF energy-based tissue fusion could accomplish fast and stable intestinal tract reconstruction,showing great potential in clinical application.It is of great significance to shorten the operation time,simplify the operation process and improve the operation quality.

关 键 词：射频能量 组织焊接 新型电极 肠道吻合 生物力学 

分 类 号：R318[医药卫生—生物医学工程]