The CRISPR/Cas9 revolution continues: From base editing to prime editing in plant science  

作　　者：Yan Li Wenjing Li Jun Li 

机构地区：[1]State Key Laboratory of North China Crop Improvement and Regulation,College of Life Sciences,Hebei Agricultural University,Baoding,Hebei 071001,China [2]College of Life Sciences,Capital Normal University,Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement,Beijing 100048,China

出　　处：《Journal of Genetics and Genomics》2021年第8期661-670,共10页遗传学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (32000454);Provincial Natural Science Foundation of Hebei for Excellent Young Scholar (C2020204062);Program for Young Talents of Hebei Education Department (BJ2021025);Starting Grant from Hebei Agricultural University (YJ201958)。

摘　　要：The ability to precisely inactivate or modify genes in model organisms helps us understand the mysteries of life. Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9), a revolutionary technology that could generate targeted mutants, has facilitated notable advances in plant science. Genome editing with CRISPR/Cas9 has gained great popularity and enabled several technical breakthroughs. Herein, we briefly introduce the CRISPR/Cas9, with a focus on the latest breakthroughs in precise genome editing(e.g., base editing and prime editing), and we summarize various platforms that developed to increase the editing efficiency, expand the targeting scope, and improve the specificity of base editing in plants. In addition, we emphasize the recent applications of these technologies to plants. Finally, we predict that CRISPR/Cas9 and CRISPR/Cas9-based genome editing will continue to revolutionize plant science and provide technical support for sustainable agricultural development.

关 键 词：CRISPR/Cas9 Base editing Prime editing LOSS-OF-FUNCTION GAIN-OF-FUNCTION DNA free 

分 类 号：Q943.2[生物学—植物学]