Sporophytic control of anther development and male fertility by glucose-6-phosphate/phosphate translocator 1(OsGPT1) in rice  

作　　者：Aili Qu Yan Xu Xinxing Yu Qi Si Xuwen Xu Changhao Liu Liuyi Yang Yueping Zheng Mengmeng Zhang Shuqun Zhang Juan Xu 

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry,College of Life Sciences,Zhejiang University,Hangzhou,Zhejiang 310058,China [2]Department of Plant Pathology,College of Plant Protection,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China [3]Division of Biochemistry,University of Missouri,Columbia,MO 65211,USA

出　　处：《Journal of Genetics and Genomics》2021年第8期695-705,共11页遗传学报（英文版）

基　　金：supported by the grants from National Natural Science Foundation of China (31922005);Zhejiang Provincial Natural Science Foundation of China (LR18C020001);Zhejiang University K.P.Chao’s High Technology Development Foundation (2018RC016);the Young Elite Scientist Sponsorship Program by CAST (2018QNRC001);111 Project (B14027) to J.X。

摘　　要：Coordination between the sporophytic tissue and the gametic pollen within anthers is tightly controlled to achieve the optimal pollen fitness. Glucose-6-phosphate/phosphate translocator(GPT) transports glucose-6-phosphate, a key precursor of starch and/or fatty acid biosynthesis, into plastids. Here, we report the functional characterization of Os GPT1 in the rice anther development and pollen fertility. Pollen grains from homozygous osgpt1 mutant plants fail to accumulate starch granules, resulting in pollen sterility. Genetic analyses reveal a sporophytic effect for this mutation. Os GPT1 is highly expressed in the tapetal layer of rice anther. Degeneration of the tapetum, an important process to provide cellular contents to support pollen development, is impeded in osgpt1 plants. In addition, defective intine and exine are observed in the pollen from osgpt1 plants. Expression levels of multiple genes that are important to tapetum degeneration or pollen wall formation are significantly decreased in osgpt1 anthers. Previously, we reported that At GPT1 plays a gametic function in the accumulation of lipid bodies in Arabidopsis pollen. This report highlights a sporophytic role of Os GPT1 in the tapetum degeneration and pollen development. The divergent functions of Os GPT1 and At GPT1 in pollen development might be a result of their independent evolution after monocots and dicots diverged.

关 键 词：Glucose-6-phosphate/phosphate TRANSLOCATOR Male fertility Starch accumulation Sporophytic control Pollen development Tapetum degeneration Intine and exine formation RICE 

分 类 号：S511[农业科学—作物学]