铝胁迫下八仙花ABC转运蛋白基因家族的鉴定与生物信息学分析  

Identification and Bioinformatics Analysis of ABC Transporter Gene Family in Hydrangea under Aluminum Stress

作　　者：陈海霞[1] 王暄 许璐 Chen Haixia;Wang Xuan;Xu Lu(Hunan Mid-subtropical Quality Plant Breeding and Utilization Engineering Technology Research Center,College of Horticulture,Hunan Agricultural University,Changsha,410128)

机构地区：[1]湖南农业大学园艺学院湖南省中亚热带优质花木繁育与利用工程技术研究中心,长沙410128

出　　处：《分子植物育种》2021年第20期6613-6623,共11页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31201656);湖南省自然科学基金项目(2017JJ3105)共同资助。

摘　　要：ABC转运蛋白是一类广泛存在于原核和真核生物中的跨膜运输蛋白,参与植物信号转导、次生代谢物运输和非生物胁迫响应等过程。本研究基于八仙花转录组数据,利用生物信息学分析方法对铝胁迫下ABC转运蛋白基因家族成员、理化性质、基因结构、系统发育以及基因表达模式进行分析。结果表明八仙花ABC转运蛋白基因家族包括48个成员,分别属于7个亚家族,其中C亚家族成员最多;亚细胞定位结果显示50%的家族成员位于质膜上;理化性质和结构域分析显示绝大多数为疏水性蛋白,不同亚家族均具有特异的保守基序,但是motif 1和motif 2存在所有八仙花ABC转运蛋白中;将拟南芥129个ABC转运蛋白构建系统发育树,这48个八仙花的ABC转运蛋白分别聚在拟南芥的7个ABC亚家族中。表达谱分析结果发现,铝胁迫处理后HmABCA1、HmABCC1、HmABCC6、HmABCC14和HmABCD1基因在根部上调表达,仅HmABCG1基因在叶片中上调表达。本研究结果为进一步研究八仙花ABC转运蛋白基因的耐铝功能和表达调控提供了参考。ABC transporters is a type of transmembrane transporters widely found in prokaryotes and eukaryotes.They are involved in plant signal transduction,secondary metabolite transport and abiotic stress response.According to the transcriptome data of Hydrangea,this study was proposed to analyze the members of ABC transporter gene family,physical and chemical properties,gene structure,phylogeny and gene expression patterns under aluminum stress using bioinformatics method.The results showed that there were 48 members of ABC transporter gene family in Hydrangea,which belonged to 7 subfamilies respectively,among which the C subfamily had the most members;The subcellular localization results showed that 50%of the family members were located on the plasma membrane;The physical and chemical properties and domain analysis showed that most of the ABC transporters were hydrophobic proteins,and different subfamilies had specific conserved motifs,but motif 1 and motif 2 were all present in Hydrangea ABC transporter;A phylogenetic tree was constructed with 129 ABC transporters of Arabidopsis thaliana,and 48 ABC transporters from Hydrangea were clustered into 7 subfamilies of Arabidopsis thaliana respectively.The expression profile analysis showed that the HmABCA1,HmABCC1,HmABCC6,HmABCC14 and HmABCD1 gene were upregulated in the root and HmABCG1 gene was up-regulated in leaves after aluminum stress treatment.The results provide a reference for further study on aluminum tolerance and expression regulation of ABC transporter genes in Hydrangea.

关 键 词：八仙花 耐铝性 ABC转运蛋白基因 生物信息学 

分 类 号：S685.99[农业科学—观赏园艺]