猪传染性胃肠炎病毒疫苗株S基因的克隆及其与流行株的比对分析  

Cloning of S Genes from Porcine Transmissible Gastroenteritis Virus Vaccine Strains and Sequence Comparison with Epidemic Strains

作　　者：侯宏艳[1] 潘孝成[1] 尹磊 张丹俊[1] 赵瑞宏[1] 胡晓苗[1] 周学利[1] HOU Hong-yan;PAN Xiao-cheng;YIN Lei;ZHANG Dan-jun;ZHAO Rui-hong;HU Xiao-miao;ZHOU Xue-li(Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering/Livestock and Poultry Epidemic Diseases Research Center of Anhui Province,Institute of Animal Husbandry and Veterinary Science,Anhui Academy of Agricultural Science,Hefei 230031,China)

机构地区：[1]安徽省农业科学院畜牧兽医研究所安徽省畜禽疫病研究中心畜禽产品安全工程安徽省重点实验室,安徽合肥230031

出　　处：《畜牧与饲料科学》2021年第5期12-15,共4页Animal Husbandry and Feed Science

基　　金：安徽省农业科学院平台项目(2021YL065)。

摘　　要：[目的]分析猪传染性胃肠炎病毒(porcine transmissible gastroenteritis virus,TGEV)疫苗株和流行株S基因的遗传变异特征。[方法]利用RT-PCR方法对国内目前广泛使用的TGEV疫苗株A、B、C的S基因进行克隆并测序,将获得的序列与近年来公开发表的分离自不同地区的TGEV毒株S基因序列进行比对分析;选取NCBI中登录号为AF104420.1-Britain、AY587882.1-Nanjing、DQ811786.2-America、EU074218.2-Harbin、FJ755618.2-Harbin、JQ700304-fuzhou、KC609371.1-Shanghai、KX900411.1-United States、M94099.1-Spain和MK272773.1-Fushun的TGEV流行株S基因序列,与疫苗株A、B、C的S基因序列一起,利用MegAlign软件进行比对分析。[结果]疫苗株A、B、C的S基因序列两两间的同源性均在96.7%以上;疫苗株A、B、C的S基因序列与NCBI中流行株S基因序列的同源性分别在94.43%~99.82%、95.30%~99.64%、93.48%~98.38%;由遗传进化树可知,疫苗株A、C与国外流行株M94099.1-Spain在同一分支上,疫苗株B与国内流行株JQ700304-fuzhou在另一分支上。[结论]TGEV疫苗株的S基因与流行株同源性较高,变异性不大。[Objective]To characterize the genetic variations of S gene in vaccine strains and epidemic strains of porcine transmissible gastroenteritis virus(TGEV).[Method]The S genes from the commonly used TGEV vaccine strains of A,B and C in China were cloned by RT-PCR assay and subsequently sequenced.The obtained sequences were genetically compared with the published S gene sequences of TGEV strains isolated from different regions in recent years.The S gene sequences from TGEV vaccine strains of A,B,C and TGEV epidemic strains published in NCBI with accession numbers of AF104420.1-Britain,AY587882.1-Nanjing,DQ811786.2-America,EU074218.2-Harbin,FJ755618.2-Harbin,JQ700304-fuzhou,KC609371.1-Shanghai,KX900411.1-United States,M94099.1-Spain and MK272773.1-Fushun were genetically assessed with MegAlign software.[Result]The homologies of S gene sequences among vaccine strains of A,B and C were all above 96.7%,and those among vaccine strains of A,B,C and the epidemic strains published in NCBI were 94.43%-99.82%,95.30%-99.64%and 93.48%-98.38%,respectively.The phylogenetic trees demonstrated that vaccine strains of A and C as well as abroad epidemic strain of M94099.1-Spain were clustered in the same branch,and vaccine strain of B and domestic epidemic strain of JQ700304-fuzhou were clustered in another branch.[Conclusion]The S gene sequences of TGEV vaccine strains have high homologies with those of TGEV epidemic strains,indicating minor genetic variations in S gene sequences between vaccine strains and epidemic strains.

关 键 词：猪传染性胃肠炎病毒 疫苗株 流行株 S基因 遗传变异 

分 类 号：S852.651[农业科学—基础兽医学]