基于GC-MS技术分析感染BmNPV家蚕幼虫脂肪体组织的代谢特征  

Analysis of Metabolic Characteristics in Fat Body of Bombyx mori Infected with Bm NPV by GC-MS Technology

作　　者：张潇 郭慧敏 张本争 赵国栋 李刚 徐安英[1,2] 钱荷英 Zhang Xiao;Guo Huimin;Zhang Benzheng;Zhao Guodong;Li Gang;Xu Anying;Qian Heying(School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212100,China;Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212100,China)

机构地区：[1]江苏科技大学生物技术学院,江苏镇江212100 [2]中国农业科学院蚕业研究所,江苏镇江212100

出　　处：《蚕业科学》2021年第4期335-344,共10页ACTA SERICOLOGICA SINICA

基　　金：财政部和农业农村部国家现代农业产业技术体系项目(CARS-18);江苏省自然科学基金面上项目(BK20181228);江苏省重点研发计划(现代农业)项目(BE2020418)。

摘　　要：选择抗脓病家蚕品种白玉N和敏感品种白玉为研究对象,运用GC-MS技术对添食接种高浓度BmNPV前后2个品种5龄幼虫的4种脂肪体组织样品进行代谢组学研究,分析受到BmNPV胁迫后脂肪体组织在代谢组水平发生的变化,筛选差异代谢物,并进一步分析差异代谢通路。结果显示:抗性品种白玉N和敏感品种白玉的幼虫脂肪体中共检出各类差异代谢物407种,主要包括氨基酸、糖类、核苷酸、醛和酮等小分子物质,其中胱氨酸、正缬氨酸、香芹酮、岩藻糖等24种代谢物特异性地出现在某一个参比样品中。根据差异代谢物进行KEGG代谢通路富集分析,再对每个参比组中差异最显著的10条代谢通路进行功能聚类,获得可能造成不同品种间抗病性迥异的3条代谢通路,即嘌呤代谢、嘧啶代谢以及泛酸和辅酶A的生物合成,这些代谢通路与家蚕的能量代谢和供给、体内DNA和RNA合成以及氮代谢密切相关。The silkworm variety Baiyu N resistant to BmNPV and Baiyu sensitive to BmNPV were selected as the research objects. The metabonomics of 4 fat body samples in two varieties of 5 th instar larvae were studied by GC-MS technology before and after feeding high concentration BmNPV. The objective was to analyze the changes of fat body tissue at metabonomic level after BmNPV stress, screen the differential metabolites, and to further analyze the differential metabolic pathway. The results showed that 407 differential metabolites were detected in larval fat body of resistant variety Baiyu N and sensitive variety Baiyu, mainly including amino acids, sugars, nucleotides, aldehydes, ketones and other small molecules. Among them, 24 metabolites appeared in a reference sample specifically, such as cystine, norvaline, carvone, fucose, etc. Enrichment analysis of KEGG metabolic pathway based on the different metabolites was carried out, and functional cluster analysis on the 10 metabolic pathways with the most significant differences in each reference group was performed. As a result, 3 metabolic pathways that may cause different disease resistances among different varieties were obtained, namely purine metabolism, pyrimidine metabolism, and pantothenate and CoA biosynthesis. These metabolic pathways were closely related to the energy metabolism and supply, DNA and RNA synthesis, and nitrogen metabolism of silkworm.

关 键 词：BMNPV GC-MS技术 家蚕脂肪体 差异代谢物 代谢通路 

分 类 号：S881.21[农业科学—特种经济动物饲养] S884.51[农业科学—畜牧兽医]