多孔转基因丝素支架的制备及性能测试  

Preparation and Properties of Porous Transgenic Silk Fibroin Scaffolds

作　　者：赵硕 叶小刚 吴美玉 阮靖华 戴湘平 王欣秋 余世华 钟伯雄[1] Zhao Shuo;Ye Xiaogang;Wu Meiyu;Ruan Jinghua;Dai Xiangping;Wang Xinqiu;Yu Shihua;Zhong Boxiong(College of Animal Sciences,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学动物科学学院,杭州310058

出　　处：《蚕业科学》2021年第4期358-363,共6页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金项目(31772676);国家自然科学基金青年基金项目(31702183)。

摘　　要：以2种融合了蜘蛛丝蛋白的转基因蚕丝为材料制备转基因丝素支架,通过傅里叶变换红外光谱(FTIR)、扫描电子显微镜(SEM)及压缩测试等方法表征转基因丝素支架的结构与性能变化。结果表明,转基因丝素支架的横截面以蜂窝状多孔结构为主,易于细胞的黏附和生长;再生丝素蛋白结构发生变化,β折叠结构含量提高了16.14%;力学性能得到改善,压缩模量和抗压强度分别提高了24.49%和38.11%。体外细胞培养验证了转基因支架材料生物相容性良好,对细胞增殖没有显著影响,是一种更理想的肌骨组织修复医用备选材料。研究证实了转基因蚕丝应用于再生丝素材料的可行性,为组织工程材料开发提供了新的方向。Transgenic silk fibroin scaffolds were prepared from two kinds of transgenic silk fused with spider silk protein. The structure and properties of the scaffolds were characterized by Fourier transform infrared spectroscopy(FTIR), scanning electron microscopy(SEM) and compression test. Results showed that the cross-section of transgenic silk fibroin scaffolds was mainly honeycomb porous structure, which was easy for cell adhesion and growth. The structure of regenerated silk fibroin was changed, and the content of β-sheet structure increased by 16.14%. The mechanical properties were improved, and the compressive modulus and compressive strength increased by 24.49% and 38.11% respectively. The in vitro cell culture experiment verified that the transgenic scaffold has good biocompatibility and did not influence cell proliferation, so it is a better medical candidate material for tissue repair. This study confirmed the feasibility of transgenic silk used in regenerated silk fibroin materials, and provided a new direction for the development of tissue engineering materials.

关 键 词：转基因蚕丝 丝素支架 蜘蛛丝蛋白 二级结构 横截面形貌 抗压强度 

分 类 号：S881.24[农业科学—特种经济动物饲养] S961.6[农业科学—畜牧兽医]