云南西北部高山流石滩不同垫状植物的微生物群落及功能类群的空间异质性  

Spatial heterogeneity of microbial community and functional groups of different cushion species in alpine scree habitat in northwestern Yunnan,China

作　　者：肖筑伟 宋敏舒[1] 周杰 石玲玲 杨扬[1] XIAO Zhuwei;SONG Minshu;ZHOU Jie;SHI Lingling;YANG Yang(Key Laboratory for Plant Diversity and Biogeography of East Asia,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650224,China;Georg August University of Göttingen,Göttingen 37077,Germany;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院昆明植物研究所,中国科学院东亚植物多样性与生物地理学重点实验室,昆明650224 [2]德国哥廷根大学,哥廷根370773 [3]中国科学院大学,北京100049

出　　处：《应用与环境生物学报》2021年第5期1119-1129,共11页Chinese Journal of Applied and Environmental Biology

基　　金：云南省应用基础研究计划项目(2018FA015);云南省野外科学观测研究站建设项目(202105AM070006)资助。

摘　　要：作为植物通过趋同进化方式适应高海拔极端生境的典型代表之一,高山垫状植物普遍发生于不同科、属的植物种类间,且因其垫状特化结构对土壤环境具有显著影响而备受关注.为深入阐述垫状植物不同代表种类和同一垫状植物不同空间内土壤微生物群落及功能类群的变化特点,选取云南西北部白马雪山高山流石滩生境内滇西北点地梅(Androsace delavayi)、团状福禄草(Arenaria polytrichoides)和粗糙红景天(Rhodiola coccinea var.scabrida)3种具有相似地上垫状形态和不同地下根系类型的垫状植物代表类群为研究对象,采用高通量测序技术,比较分析垫状植物茎际(地上垫状结构内部)、边缘(地上垫状结构边缘)、根际和裸地4种空间位置的土壤微生物群落.结果显示:(1)微生物群落丰富度(观测物种数)和多样性(香农指数)在不同垫状植物相同空间位置间的差异显著,而在同一垫状植物不同空间的差异不显著,其中粗糙红景天的4种空间中的微生物群落丰富度最高,而滇西北点地梅根际微生物群落丰富度和多样性最低;(2)土壤pH值和植物根长与微生物群落多样性呈显著相关(Pearson相关分析);(3)不同物种相同空间位置和同一物种不同空间位置中微生物群落的组成、优势类群和功能类群均差异显著,但其中与氮循环相关的类群(如硝化细菌,反硝化细菌,还原硝酸盐类群等)在每一个样本的土壤群落组成上都占了较高的比例,潜在指示了微生物对垫状植物养分吸收的可能协助作用.本研究证实垫状植物微生物群落具有显著物种差异性和空间异质性的特点,为进一步认知和理解高山流石滩极端环境中植物与微生物的共生关系及其生态适应意义和功能提供了新的研究资料与证据.Cushion species,one of the most pronounced examples of the highly specialized morphology occurring in systematically distant plant species in alpine areas,are well documented for their incredible capacity to modify soil conditions.They can affect both abiotic factors(e.g.,water and nutrient contents)and biotic factors(e.g.,microbial community diversity,composition,and structure).However,the spatial distributions of microbial communities and their functional groups among different plant species sharing similar cushion morphologies and their correlations with above-and belowground plant traits and soil chemical characteristics remain largely unstudied.In this work,we selected three typical cushion plant species(i.e.,Androsace delavayi,Arenaria polytrichoides,and Rhodiola coccinea var.scabrida)that occur in the alpine scree habitat of Baima Snow Mountain,Deqen County,northwestern Yunnan Province,and examined the microbial community of soil samples collected at four different spatial locations therein:inside and at the edge of the aboveground cushion structure,the rhizosphere,and surrounding bare ground uncovered by any cushion species using highthroughput sequencing technology.The main findings were as follows.(1)There were significant differences in microbial community richness(observed species)and diversity(Shannon index)at the same spatial location among different cushion species but no significant spatial differences among each of these.Of the three cushion species studied,R.coccinea var.scabrida exhibited the greatest microbial community richness at all four spatial locations,while the microbial community richness and diversity in the rhizosphere of Androsace delavayi were the lowest.(2)In terms of the microbial community diversity,there was a significantly positive relationship soil pH and root length.Finally,(3)the microbial community exhibited clear species-specific and spatial differences in their compositions,dominant taxa,and functional groups,with a great proportion of some bacteria in nitrogen cycling g

关 键 词：土壤微生物 微生物功能类群 高山流石滩 垫状植物 高通量测序 

分 类 号：S154.3[农业科学—土壤学]