贡嘎山峨眉冷杉根际、非根际土壤细菌群落的时空分布及影响因素  

Spatiotemporal distribution patterns and drivers of bacterial communitiesin the rhizosphere and bulk soil under an Abies fabri forest onGongga Mountain

作　　者：熊文君 祝贺[3] 李家宝[1] 谢婷 吴艳宏[3] 李香真[1] XIONG Wenjun;ZHU He;LI Jiabao;XIE Ting;WU Yanhong;LI Xiangzhen(Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China;University of Chinese Academy of Sciences,Beijing 100049,China;Chengdu Institute of Mountain Hazard and Environment,Chinese Academy of Sciences,Chengdu 610041,China)

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]中国科学院大学,北京100049 [3]中国科学院成都山地灾害与环境研究所,成都610041

出　　处：《应用与环境生物学报》2021年第5期1130-1137,共8页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金面上项目(31870473)和重点项目(41630751)资助

摘　　要：土壤细菌群落的时空动态格局和驱动因素一直是山地森林生态系统关注的重点,其中海拔梯度上表层土壤细菌群落的多样性分布模式及驱动因素为研究热点,但关于植物根际、非根际微生物随海拔和季节变化规律的研究还比较缺乏.于2017年5月、8月、10月沿贡嘎山东坡2800 m、3000 m、3200 m、3500 m的4个海拔,采集峨眉冷杉根际与非根际土壤,利用Miseq测序技术和生物信息学方法,研究根际、非根际土壤细菌组成和多样性的时空变异及其影响因素.结果显示,细菌群落中相对丰度含量最高的前9个菌属为变杆菌属(Variibacter,相对丰度为5.5%)、热酸菌属(Acidothermus,4.2%)、阿菲波菌(Afipia,2.5%)、单胞菌属(Bryobacter,2.5%)、嗜酸铁-γ变形杆菌(Acidibacter,1.6%)、副球菌属(Isosphaera,1.5%)、Rhizomicrobium(1.2%)、Candidatus soilbacter(0.05%)和Granulicella(0.02%).海拔梯度上,冷杉根际和非根际土壤细菌群落的α多样性呈现单峰模式,在3200 m处达到峰值.细菌群落的α多样性与土壤pH之间存在显著的相关关系(r=0.81,P=0.001).此外,并未观察到该森林土壤中根际与非根际间的细菌群落结构有显著差异.本研究揭示了山地生态系统中冷杉根际、非根际细菌群落在组成和多样性的时空变异及重要驱动因子,可为完善山地森林生态系统中细菌分布格局提供数据支撑.The study of spatial patterns in soil microbial communities in mountain ecosystems has received much attention.However,elevational and seasonal patterns and environmental drivers of bacterial communities associated with the plant rhizosphere remain understudied.In this work,we collected 72 soil samples from a subalpine dark coniferous forest at four elevations on Gongga Mountain in May,August,and October 2017.We then studied the distribution patterns and drivers of soil bacterial community composition and diversity using MiSeq sequencing.The results indicated that bacteria in the genera Variibacter(5%),Acidothermus(4.2%),Afipia(2.5%),Bryobacter(2.5%),Acidibacter(1.6%),Isosphaera(1.5%),Rhizomicrobium(1.2%),and Granulicella(0.02%),as well as the species Candidatus Soilbacter(0.05%),were dominant in both the Abies fabri rhizosphere and bulk soil.Bacterial a diversity in both types of soil increased from 2800 m to 3200 m and decreased from 3200 m to 3500 m.There was a significant correlation between theαdiversity of bacterial community and soil pH(r=0.81,P=0.001).In addition,there was no significant difference in the structure of the bacterial community in the rhizosphere and bulk soil.Overall,our findings revealed the spatiotemporal dynamics of bacteria associated with plant root in subalpine forest soils and may provide data-based support for a comprehensive understanding of microbial distribution in this ecosystem.

关 键 词：山地生态系统 峨眉冷杉 根际土壤 非根际土壤 海拔 季节 细菌群落 

分 类 号：S714.3[农业科学—林学]