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作 者:宋欣 孙士权[1,2,3] 商光琦 张伟 张允宝 蒋昌波 万俊力[1,2,3] 吴方同 陈婧[1,2,3] 谭万春 SONG Xin;SUN Shi-quan;SHANG Guang-qi;ZHANG Wei;ZHANG Yun-bao;JIANG Chang-bo;WAN Jun-li;WU Fang-tong;CHEN Jing;TAN Wan-chun(School of Hydraulic Engineering,Changsha University of Science&Technology,Changsha 410114,China;Key Laboratory of Dongting Lake Aquatic Eco-Environmental Control and Restoration of Hunan Province,Changsha 410114,China;Engineering and Technical Center of Hunan Provincial Environmental Protection for River-Lake Dredging Pollution Control,Changsha 410114,China;Transportation Construction Cost Management Station of Hunan Transportation Department,Changsha 410016,China)
机构地区:[1]长沙理工大学水利工程学院,湖南长沙410114 [2]洞庭湖水环境治理与生态修复湖南省重点实验室,湖南长沙410114 [3]湖南省环境保护河湖疏浚污染控制工程技术中心,湖南长沙410114 [4]湖南省交通运输厅交通建设造价管理站,湖南长沙410016
出 处:《中国给水排水》2021年第19期91-97,共7页China Water & Wastewater
基 金:国家自然科学基金资助项目(51309032);湖南省交通运输厅科技进步与创新项目(201802);湖南省教育厅优秀青年基金资助项目(17B014);水文水资源与水利工程科学国家重点实验室项目(2016490911);湖南省研究生科研创新项目(CX2018B554);湖南省重点领域研发项目(2019SK2191)。
摘 要:以絮状污泥作为接种污泥,通过改变进水氮硫比(N∶S)培养好氧颗粒污泥,考察其对污泥颗粒化的影响及反应器内COD、氮、磷浓度的变化,并利用微生物基因测序手段分析微生物群落结构的变化。结果表明,当N∶S为2∶1和4∶1时,污泥处于连续膨胀状态,无法形成颗粒,此时MLSS为1.104~3.647 g/L、SVI_(5)为137~416 mL/g、SV_(5)为40%~60%。当N∶S升至8∶1时,污泥流失得到控制,并在第72天时培养出成熟的好氧颗粒污泥,其粒径主要分布在0.4~0.8 mm,此时MLSS为3.27~5.17 g/L、SVI_(5)为36~67 mL/g、SV_(5)为12%~20%。污泥颗粒化后,对COD、NH_(4)^(+)-N、TN、TP的平均去除率分别达到95.51%、99.62%、61.30%、25.62%。而整个培养过程中,对硫化物的去除率可达到99%。对成熟颗粒污泥进行微生物群落多样性检测,结果表明,颗粒污泥中Paracoccus、Meganema、Azoarcus为优势菌属,隶属于变形菌门。Aerobic granular sludge was cultivated by inoculating flocculent sludge and changing the influent nitrogen to sulfur ratio(N∶S).Effects of nitrogen to sulfur ratio on aerobic granulation and variation of COD,nitrogen and phosphorus concentrations in the reactor were investigated,and the changes of the microbial community composition were analyzed by microbial genetic sequencing.When the N∶S was 2∶1 and 4∶1,the sludge in the reactor was in a continuous bulking state and could not granulate.At this time,MLSS was 1.104-3.647 g/L,SVI_(5)was 137-416 mL/g,and SV_(5) was 40%-60%.When the N∶S increased to 8∶1,the sludge loss was controlled,and the mature aerobic granular sludge with the particle size in the range of 0.4-0.8 mm was appeared on the 72 nd day.At this time,MLSS was 3.27-5.17 g/L,SVI_(5)was 36-67 mL/g,and SV_(5) was 12%-20%.After granulation,the average removal efficiencies of COD,NH_(4)^(+)-N,TN and TP were 95.51%,99.62%,61.30%and 25.62%,respectively.However,the removal efficiency of sulfide reached 99%during the cultivation.The microbial community diversity of mature granular sludge was detected,and Paracoccus,Meganema and Azoarcus belonging to Proteobacteria were the dominant bacteria.
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