Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography  

作　　者：Zhe Wang Vittorio Bianco Daniele Pirone Pasquale Memmolo Massimiliano Maria Villone Pier Luca Maffettone Pietro Ferraro 

机构地区：[1]Dipartimento di Ingegneria Chimica dei Materiali e della Produzione Industriale,Universitàdegli Studi di Napoli“Federico II”,Piazzale Tecchio 80,80125 Napoli,Italy [2]NEAPoLIS,Numerical and Experimental Advanced Program on Liquids and Interface Systems,Joint Research Center CNR-Universitàdegli Studi di Napoli“Federico II”,Napoli,Italy [3]CNR-ISASI,Institute of Applied Sciences and Intelligent Systems“E.Caianiello”,Via Campi Flegrei 34,80078 Pozzuoli,Napoli,Italy [4]Dipartimento di Ingegneria Elettrica e delle Tecnologie dell’Informazione,Universitàdegli Studi di Napoli“Federico II”,via Claudio 21,80125 Napoli,Italy

出　　处：《Light(Science & Applications)》2021年第10期1889-1904,共16页光（科学与应用)（英文版）

摘　　要：Single-cell phase-contrast tomography promises to become decisive for studying 3D intracellular structures in biology.It involves probing cells with light at wide angles,which unfortunately requires complex systems.Here we show an intriguing concept based on an inherent natural process for plants biology,i.e.,dehydration,allowing us to easily obtain 3D-tomography of onion-epidermal cells’nuclei.In fact,the loss of water reduces the turgor pressure and we recognize it induces significant rotation of cells’nuclei.Thanks to the holographic focusing flexibility and an ad-hoc angles’tracking algorithm,we combine different phase-contrast views of the nuclei to retrieve their 3D refractive index distribution.Nucleolus identification capability and a strategy for measuring morphology,dry mass,biovolume,and refractive index statistics are reported and discussed.This new concept could revolutionize the investigation in plant biology by enabling dynamic 3D quantitative and label-free analysis at sub-nuclear level using a conventional holographic setup.

关 键 词：ROTATION HOLOGRAPHIC phase 

分 类 号：O57[理学—粒子物理与原子核物理]