五丹胃福颗粒对胃癌前病变大鼠组织病理学及VEGF和HIF-1α的影响研究  

Effects of Wudan Weifu Granule on Histopathology,VEGF and HIF-1α in Rats with PLGC

作　　者：祁向争[1] 胡建功[1] 马志豪 李志强 姬壮壮 徐旭 QI Xiangzheng;HU Jiangong;MA Zhihao;LI Zhiqiang;JI Zhuangzhuang;XU Xu(The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300150,China;Tianjin Pharmaceutical Research Institute Co.,Ltd.,Tianjin 300193,China)

机构地区：[1]天津中医药大学第二附属医院,天津300150 [2]天津药物研究院有限公司,天津300193

出　　处：《中医药学报》2021年第11期28-32,共5页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(81704003)。

摘　　要：目的:通过观察大鼠胃黏膜组织病理及黏膜中VEGF和HIF-1α的表达,探讨五丹胃福颗粒对PLGC大鼠的干预机制。方法:采用改良MNNG+复合法造模成功PLGC大鼠56只,随机分为模型组(A组)、五丹胃福低剂量组(B组)、五丹胃福中剂量组(C组)、五丹胃福高剂量组(D组)、理气组(E组)、祛瘀组(F组)、叶酸组(G组)。治疗12周后,采用Western Blot法检测各组VEGF、HIF-1α表达水平;采用HE染色观察各组大鼠黏膜组织病理学。结果:VEGF:治疗12周,与A组比较,其他组胃窦部黏膜VEGF显著降低(P<0.05)。B组、C组与D组比较,差异无统计学意义(P>0.05)。E组、F组与D组相比,差异无统计学意义(P>0.05),但VEGF降低趋势在F组比E组更明显。E组与F组比较,差异没有统计学意义(P>0.05)。HIF-1α:治疗12周,与A组比较,其他组胃窦部黏膜HIF-1α显著降低(P<0.05);B组、C组与D组相比,差异无统计学意义(P>0.05);E组、F组与D组相比,差异没有统计学意义(P>0.05),但HIF-1α降低趋势在E组比F组更明显。E组与F组比较,差异没有统计学意义(P>0.05)。病理:各组间炎症积分比较:B组、C组、D组、G组与A组比较,炎症积分减低(P<0.05)。B组、C组与D组比较,P>0.05。各组间萎缩程度积分比较:B组、C组、D组与A组比较,积分减低(P<0.05)。B组、C组与D组比较,P>0.05。各组间瘤变程度积分比较:D组、G组与A组比较,积分减低(P<0.05)。结论:五丹胃福颗粒通过降低PLGC大鼠的VEGF及HIF-1α表达,从而使胃黏膜病理积分显著降低,延缓了PLGC向胃癌的进展。Objective:To investigate the intervention mechanism of Wudan Weifu Granule in rats with precancerous lesion of gastric cancer(PLGC)by observing the pathology of gastric mucosa and the expressions of VEGF and HIF-1αin mucosa.Methods:56 PLGC rats were successfully modeled by the modified MNNG plus compound method and divided into the model group(group A),Wudan Weifu Granule groups of low-dose(group B),medium-dose(group C)and high-dose(group D),Qi regulating group(group E),Quyu group(group F)and folic acid group(group G).After 12 weeks of the treatment,the expression levels of VEGF and HIF-1αwere detected by Western Blot method and the mucosal histopathology were observed in each group.Results:In terms of the expression of VEGF,it was significant lower in group B,group C,group D,group E,group F and group G than that in group A after 12 weeks of the treatment(P<0.05);there was no statistical difference among group B,group C and group D(P>0.05);compared to that in group D,it did not show significant difference in group E and group F(P>0.05),but the reduction trend was more obvious in group F than that in group E;there was no statistical difference between group E and group F(P>0.05).In terms of the expression of HIF-1α,it was significant lower in group B,group C,group D,group E,group F and group G compared to that in group A after 12 weeks of the treatment(P<0.05);there was no statistical difference among group B,group C and group D(P>0.05);compared to that in group D,it did not show significant difference in group E and group F(P>0.05);the decrease of HIF-1αwas more obvious in group E than that in group F,but there was no statistical difference between the two groups(P>0.05).In terms of the pathology changes,the inflammation scores were significant decreased in group B,group C,group D and group G compared to those in group A(P<0.05);there were no statistical differences in inflammation scores among group B,group C and group D(P>0.05).The atrophy scores were significantly reduced in group B,group C and group D com

关 键 词：五丹胃福颗粒 胃癌前病变 气滞络瘀 

分 类 号：R285.5[医药卫生—中药学]