长雄野生稻与栽培稻种间杂种胚拯救研究初报  

Preliminary reports on embryo rescue of interspecific hybrid between Oryza longistaminata and cultivated rice

作　　者：査中萍[1] 郑兴飞 周黎[1] 王红波 殷得所[1] 胡建林 董华林[1] 游艾青[1] 徐得泽[1] ZHA Zhong-ping;ZHENG Xing-fei;ZHOU Li;WANG Hong-bo;YIN De-suo;HU Jian-lin;DONG Hua-lin;YOU Ai-qing;XU De-ze(Food Crops Institute,Hubei Academy of Arricultural Sciences/Hubei Key laouatory of Food Crop Germplasm and Genetic Improvement,Wuhan 430064,China)

机构地区：[1]湖北省农业科学院粮食作物研究所/粮食作物种质创新与遗传改良湖北省重点实验室,武汉430064

出　　处：《湖北农业科学》2021年第21期30-32,共3页Hubei Agricultural Sciences

基　　金：湖北省农业科学院青年科学基金项目(2020NKYJJ01);湖北省粮食作物种质创新与遗传改良重点实验室开放课题(2018lzjj03);湖北省重点研发计划项目(2020BBB053)。

摘　　要：以优良栽培稻恢复系为母本,与长雄野生稻杂交,在授粉后第7~9天对杂交后的幼胚进行离体培养。一共接种幼胚450枚,获得发育良好健壮的绿苗438株,胚拯救效率为97.3%。20 d后待组培苗约8~10 cm时,通过室内炼苗再将幼苗移入育秧盘中,10 d后统计移栽的438株幼苗,共存活434株,成活率达99.1%。Hybrid fine cultivated rice restorer lines as female parent with Oryza longistaminata,young hybridized embryos 7~9 days after pollination were cultured in vitro.A total of 450 young embryos were inoculated,and a total of 438 well-developed and robust green seedlings were obtained.The embryo rescue efficiency was 97.3%.After 20 days,when the tissue culture seedlings were about 8~10 cm,the seedlings were transferred to the seedling tray through indoor hardening.10 days later,438 seedlings were transplanted,and 434 survived with a survival rate of 99.1%.

关 键 词：野生稻 栽培稻 中间杂种 胚拯救 

分 类 号：S330[农业科学—作物遗传育种] S511.5[农业科学—农艺学]