绞股蓝多糖对D-半乳糖致衰小鼠肾脏组织结构的影响  

Effect of Gynostemma pentaphyllum polysaccharide on renal tissue structure of aging mice induced by D-galactose

作　　者：刘新勇 黎木兰 黄嘉琳 王欣雨 唐小川 王晓丽[1] LIU Xin-yong;LI Mu-lan;HUANG Jia-lin;WANG Xin-yu;TANG Xiao-chuan;WANG Xiao-li(College of Animal Science and Technology,Guangxi University,Nanning 530005,China;Nanning Animal Disease Prevention and Control Centre,Nanning 530001,China)

机构地区：[1]广西大学动物科学技术学院,南宁530005 [2]南宁市动物疫病预防控制中心,南宁530001

出　　处：《湖北农业科学》2021年第21期94-96,102,共4页Hubei Agricultural Sciences

基　　金：广西自然科学基金项目(2016GXNSFAA380039;2018GXNSFAA281020)。

摘　　要：为探究绞股蓝多糖(Gynostemma pentaphyllum polysaccharide,GPP)对D-半乳糖(D-galactose,Dgal)致衰老小鼠肾脏组织结构的影响,采用石蜡切片、HE染色法,同时结合光学显微镜观察并对肾脏中肾小球直径和肾小囊宽度进行测量统计分析。结果发现,D-gal组肾小球明显萎缩,直径变小,肾小囊腔变大,肾小体周围肾小管管腔明显,管壁上皮细胞界限不清,模糊。GPP各剂量组均能较明显地改善肾脏的结构损伤,肾小球形态趋于正常,周围的肾小管结构明显恢复。统计分析发现,GPP各浓度组的肾小球直径均显著高于D-gal组(P<0.05),各多糖组间肾小球直径差异均不显著。GPP 50 mg(/kg·d)组和100 mg(/kg·d)组与D-gal组相比,肾小囊宽度减小,差异显著(P<0.05)。结果表明,GPP对D-gal致衰老小鼠肾脏损伤有所恢复,结构形态趋于正常;GPP可以使肾小球直径、肾小囊宽度有所改善,说明GPP对D-gal致衰老小鼠的肾脏组织结构有一定的保护作用。In order to investigate the effect of Gynostemma pentaphyllum polysaccharide(GPP)on the renal tissue structure of aging mice induced by D-galactose(D-gal),paraffin section and HE staining were used to observe and analyze the glomerular diameter and renal capsule width in kidney.The results showed that in D-gal group,the glomeruli were significantly atrophied,the diameter of glomeruli became smaller,the lumen of renal tubules around the renal corpuscle was obvious,and the boundary of epithelial cells on the wall was unclear.GPP groups could significantly improve the structure of kidney damage,glomerular morphology tends to normal,the structure of renal tubules around the recovery.Statistical analysis showed that the glomerular diameter of GPP groups was significantly higher than that of D-gal group(P<0.05),and there was no significant difference in glomerular diameter among the GPP groups.Compared with D-gal group,the width of renal capsule in 50 mg(/kg·d)group and 100 mg(/kg·d)group was significantly decreased(P<0.05).It turned out that GPP could recover the kidney injury induced by D-gal,and the structural morphology tended to be normal;the diameter of glomerulus and the width of renal capsule could be improved,which indicated that GPP had a certain protective effect on the renal tissue structure of aging mice induced by D-gal.

关 键 词：绞股蓝多糖(Gynostemma pentaphyllum polysaccharide) 肾脏 衰老 D-半乳糖 小鼠 

分 类 号：S859.4[农业科学—临床兽医学]