基于UPLC-MS/MS技术的张氏消肿定痛膏物质基础研究  

Material Basis Reseach of Zhang’s Antiswelling and Relieving Pain Ointment Based on UPLC-MS/MS

作　　者：夏丽琼 徐长琼 任秋芸 梅君 黎春辉 黎珊 唐奇 黄会保 李然 XIA Liqiong;XU Changqiong;REN Qiuyun;MEI Jun;LI Chunhui;LI Shan;TANG Qi;HUANG Huibao;LI Ran(Pharmacy Department,Yueyang Hospital of Traditional Chinese Medicine,Yueyang,Hunan 414000 China;Scientific Research Institute,The Maternal and Child Health Hospital of Yueyang,Yueyang,Hunan 414000 China;Medicine College,Shantou University,Shantou,Guangdong 515000 China)

机构地区：[1]岳阳市中医医院药剂科,湖南岳阳414000 [2]岳阳市妇幼保健院科研所,湖南岳阳414000 [3]汕头大学医学院,广东汕头515000

出　　处：《湖南中医药大学学报》2021年第11期1705-1710,共6页Journal of Hunan University of Chinese Medicine

基　　金：湖南省临床医疗技术创新引导项目(2018SK52301);湖南省临床医疗技术示范基地项目(2020SK4025);岳阳市基础研究项目(201834);岳阳市科技研发指导性计划项目(201820)。

摘　　要：目的研究了解张氏消肿定痛膏的药效物质基础。方法基于液相色谱-串联质谱(UPLC-MS/MS)技术研究张氏消肿定痛膏中化学成分,并进行定性和相对含量分析。结果基于广泛靶向代谢组学技术,检测到张氏消肿定痛膏中有542个化合物,其中种类最多的是黄酮类化合物122种,其次为酚酸77种;脂质的相对百分含量最高,为(24.01±4.23)%,黄酮类次之,为(17.95±3.01)%。结论基于UPLC-MS/MS检测平台和MWDB数据库,能简单、高效检测到张氏消肿定痛膏的化学物质基础,为进一步明确其活性成分提供参考。Objective To study the material basis of Zhang’s antiswelling and relieving pain ointment.Methods Based on liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)technique,the chemical constituents in Zhang’s antiswelling and relieving pain ointment were studied,and the qualitative and relative percentage analysis were conducted.Results UPLC-MS/MS detected 542 compounds were detected in Zhang’s antiswelling and relieving pain ointment,among which 122 kinds of flavonoids were the most,followed by 77 kinds of phenolic acids,and the relative percentage of lipids were the highest(24.01±4.23)%,followed by(17.95±3.01)%of flavonoids.Conclusion Based on the UPLC-MS/MS technique,the chemical substance base of Zhang's antiswelling and relieving pain ointment can be detected simply efficiently,which can provide reference for further defining its active ingredients.

关 键 词：张氏消肿定痛膏 超高效液相色谱-串联质谱 黄酮 酚酸 脂质 

分 类 号：R284.1[医药卫生—中药学]