穗发芽抗性相关分子标记在优质小麦中的有效性验证  

Validation of Molecular Markers Related to Pre‑harvest Sprouting Resistance in High‑Quality Wheat Varieties

作　　者：李亚青[1,2] 张楠 彭义峰[1,2] 张士昌 李孟军[1,2] LI Yaqing;ZHANG Nan;PENG Yifeng;ZHANG Shichang;LI Mengjun(Shijiazhuang Academy of Agriculture and Forestry Sciences,Shijiazhuang 050041,China;Wheat Engineering Research Center of Hebei Province,Shijiazhuang 050041,China)

机构地区：[1]石家庄市农林科学研究院,河北石家庄050041 [2]河北省小麦工程技术研究中心,河北石家庄050041

出　　处：《河南农业科学》2021年第10期18-26,共9页Journal of Henan Agricultural Sciences

基　　金：石家庄市科学技术研究与发展计划项目(191490332A);河北省重点研发计划项目(20326346D)。

摘　　要：利用42个优质小麦(Triticum aestivum)品种籽粒发芽指数对6个小麦穗发芽抗性相关基因[Tamyb10、TaDFR(Dihydroflavone reductase)、TaVp-1(Viviparous‑1)、TaSdr(Seed dormancy)、TaPM19-A1(Plasma membrane 19‑A1)、TaMFT(Mother of FT and TFL1)]的分子标记的有效性进行验证,以期为抗穗发芽优质小麦品种的筛选及选育奠定基础。结果表明,小麦穗发芽抗性相关基因Tamyb10、TaDFR、TaVp-1、TaSdr、TaPM19-A1、TaMFT在42个优质小麦品种中均检测到2种等位变异,优异等位基因所占比例存在明显差异,介于4.8%~78.6%,未发现TaVp-1的TaVp-1 Bb基因型。等位基因类型与籽粒发芽指数相关性分析表明,标记myb10D、MFT-3A和MFT-A2与优质小麦穗发芽抗性极显著相关,而标记DFR-B、Vp1B3、Sdr2A、Sdr2B和PM19-A1与优质小麦穗发芽抗性相关性不显著。STS标记myb10D可用于红粒优质小麦的穗发芽抗性筛选,CAPS标记MFT-3A和STS标记MFT-A2可用于白粒优质小麦的穗发芽抗性筛选,TaMFT可能在优质小麦抗穗发芽机制中发挥着重要作用。The molecular markers of six wheat(Triticum aestivum)genes[Tamyb10,TaDFR(dihydroflavone reductase),TaVp‑1(Viviparous‑1),TaSdr(seed dormancy),TaPM19‑A1(plasma membrane 19‑A1)and TaMFT(mother of FT and TFL1)]related to pre‑harvest sprouting resistance was validated using seed germination indices of 42 high‑quality wheat varieties,so as to lay a foundation for the selection and breeding of high quality wheat varieties resistant to panicle germination.The results showed that two allelic variations for all the six genes(Tamyb10,TaDFR,TaVp‑1,TaSdr,TaPM19‑A1 and TaMFT)was identified in 42 high‑quality wheat varieties,and the proportion of favourite alleles was obviously different,which ranged from 4.8%to 78.6%,but the Vp‑1 Bb allele was not detected.The correlation analysis between allelic variations and germination indices showed that myb10D,MFT‑3A and MFT‑A2 were significantly correlated with pre‑harvest sprouting resistance,but the correlations between DFR‑B,Vp1B3,Sdr2A,Sdr2B,PM19‑A1 and pre‑harvest sprouting resistance were not significant.The STS marker myb10D could be applied in pre‑harvest sprouting resistance screening of red‑grain high‑quality wheat varieties,while the CAPS marker MFT‑3A and the STS marker MFT‑A2 could be applied in pre‑harvest sprouting resistance screening of white‑grain high‑quality wheat varieties.TaMFT maybe plays an important role in pre‑harvest sprouting resistance mechanism of high‑quality wheat varieties.

关 键 词：优质小麦 穗发芽抗性相关分子标记 穗发芽抗性相关基因 等位变异 发芽指数 

分 类 号：S512.1[农业科学—作物学]