土壤环境中可降解丙酸细菌的筛选和鉴定  

Isolation and Identification of Soil Bacterial Strains Metabolizing Propionic Acid

作　　者：都萃颖 卢佳熠 胡浪 姚国新[1] 戴余军[1] 李静[1] 郑操 DU Cuiying;LU Jiayi;HU Lang;YAO Guoxin;DAI Yujun;LI Jing;ZHENG Cao(College of Life Science and Technology,Hubei Engineering University/Hubei Province Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients,Xiaogan 432000,China)

机构地区：[1]湖北工程学院生命科学技术学院/湖北省植物功能成分利用工程技术研究中心,湖北孝感432000

出　　处：《河南农业科学》2021年第10期69-75,共7页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31900062,31700069);湖北省技术创新专项(重大项目)(2019ABA117)。

摘　　要：分离土壤中能够降解丙酸的细菌菌株,以丰富对自然界中参与丙酸代谢细菌种类的科学认识。采用土样预培养法、含丙酸唯一碳源固体培养基培养法,结合形态学观察和16S rDNA基因序列同源性分析,筛选和鉴定能够分解代谢丙酸的细菌菌株,并利用生物信息学分析筛选到的菌株中丙酸代谢路径2-甲基柠檬酸循环关键酶基因的分布情况。结果表明,共分离得到10株能够利用丙酸碳源的细菌菌株。经鉴定,该10株细菌分别为芽孢杆菌属(Bacillus)3株,红球菌属(Rhodococcus)2株,节杆菌属(Arthrobacter)、链霉菌属(Streptomyces)、微杆菌属(Microbacterium)、白蚁菌属(Isoptericola)和解木聚糖微菌属(Xylanimicrobium)各1株;且其代谢丙酸的能力具有显著差异。通过生物信息学分析,发现绝大部分菌株含有2-甲基柠檬酸循环关键酶基因,且关键酶基因的排列方式在不同类别细菌中不一致。Isolation and identification of soil bacterial strains that metabolize propionic acid can enrich the scientific understanding of the bacterial types which are able to metabolize propionic acid in nature.Through soil sample pre‑culture,solid plate culture by using propionic acid as the sole carbon source,aswell as morphological observation and the 16S rDNA sequences homologous analysis methods,the targetbacterial strains that were capable of metabolizing propionic acid were isolated and identified.Further,the key enzyme genes of 2‑methylcitrate cycle,the propionic acid metabolizing pathway,in the obtained target strains were analyzed by bioinformatics method.A total of 10 target bacterial strains were isolated and successfully classified:3 strains of Bacillus genus,2 strains of Rhodococcus genus,and 1 strain each for Arthrobacter genus,Streptomyces genus,Microbacterium genus,Isoptericola genus,and Xylanimicrobium genus.Besides,the ability of these bacteria to metabolize propionic acid was significantly different.Bioinformatics analysis showed that most of the strains contained the key enzyme genes of 2‑methylcitrate cycle,and the arrangement of the key enzyme genes was not consistent in different types of bacteria.

关 键 词：丙酸 细菌 土壤 分离鉴定 2-甲基柠檬酸循环 

分 类 号：S154.39[农业科学—土壤学]