不同光照强度对糜子叶色黄化突变体幼苗的影响  

Effects of different illumination intensities on yellow leaf mutant seedlings of broomcorn millet

作　　者：靳丰璐 陈利青[1] 张晋丽 王俊杰[1] 李红英[1] 韩渊怀[1] 张彬[1] Jin Fenglu;Chen Liqing;Zhang Jinli;Wang Junjie;Li Hongying;Han Yuanhuai;Zhang Bin(College of Agriculture,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《山西农业大学学报（自然科学版）》2021年第5期26-34,共9页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(31971906,31601369);山西省优秀青年项目(201901D211382);山西农业大学科技创新育种基金(20132-23)。

摘　　要：[目的]本文旨在调查不同光照强度下野生型和黄化突变体糜子苗期的农艺性状,测定其光合色素与关键叶绿体前体物质,在生理生化水平进一步分析光照强度对黄化突变体的影响,研究促进叶色突变体的机制。[方法]以野生型糜子YX⁃0和经甲基磺酸乙酯(EMS)诱变获得的黄化突变体YX⁃yl为研究材料,出苗后进行不同光照处理,调查其苗期的农艺性状,并测定苗期叶片的光合色素含量和关键叶绿素前体物质含量。[结果]不同光照处理下,相较于野生型糜子,黄化突变体植株矮小,叶片长度较短;12000 lx和14400 lx光照强度下,黄化突变体的叶长显著高于9600 lx的叶长,而黄化突变体的叶宽显著变窄(P<0.05);叶绿素a、叶绿素b和类胡萝卜素含量均低于野生型;黄化突变体原卟啉Ⅸ、镁原卟啉和原脱植基叶绿素含量均显著低于野生型(P<0.05),增强光照强度可促进部分叶绿素前体物质的合成。[结论]突变体叶片黄化可能是叶绿素合成受阻、整个合成途径合成能力降低导致的,在胆色素原与原卟啉转化阶段受阻使黄化突变体中叶绿素含量降低,但适当提高光照强度,可能减缓叶绿素合成受阻。[Objective]The study was proposed to investigate the agronomic traits of wild type and yellowing mutant broomcorn millet under different light intensities,to determine the contents of photosynthetic pigments and key chlorophyll precursors in the seedlings,and further to analyze the effects of illumination intensity on the yellow mutants and underlying mechanism at physiological and biochemical levels.[Methods]In the experiment,wild-type broomcorn millet YX0 and yellowing mutant YXyl obtained by ethyl methane sulfonate(EMS)mutagenesis were used as research materials.After seedling emergence,different light intensity treatments were carried out,and the agronomic characteristics of the seedlings were investigated.The photosynthetic pigment contents and the key chlorophyll precursor contents in leaves at seedling stage were determined.[Results]The results indicated that both the yellowing mutant plants and the leaf length were shorter than that of the wild type under different light treatments.Under the illumination intensities of 12000 lx and 14400 lx,the leaf lengths of the yellowing mutant were significantly higher than that of 9600 lx treated leaves,while the leaf widths of the yellowing mutant were significantly narrower(P<0.05).The contents of chlorophyll a,chlorophyll b and total carotenoid were all lower than the wild type.The protoporphyrinⅨ(ProtoⅨ),Mg-protoⅨ(Mg-protoⅨ)and protochlorophyll(Pchlide)contents of yellowing mutant were significantly lower than those of the wild type(P<0.05).Enhanced illumination intensity could promote the synthesis of some chlorophyll precursors.[Conclusion]The yellowing of mutant leaves might be induced by the obstruction of chlorophyll synthesis and the reduction of the synthesis ability of the whole synthesis pathway.The hindering of the conversion of porphobilinogen(PBG)and protoporphyrinⅨ(ProtoⅨ)reduced the chlorophyll contents in yellowing mutant,but appropriately increasing the illumination intensity may slow down the obstruction of chlorophyll synthesis.

关 键 词：光照强度 糜子 黄化突变体 叶绿素含量 叶绿素前体 

分 类 号：S516[农业科学—作物学]