红毛七中一个新的羽扇豆烷型三萜皂苷  

A new lupane-type triterpenoid saponin from Caulophyllum robustum

作　　者：刘渝 汤海峰 王玉梅[3] 陆云阳 张倩 刘杨 毕琳琳 LIU Yu;TANG Hai-feng;WANG Yu-mei;LU Yun-yang;ZHANG Qian;LIU Yang;BI Lin-lin(Procurement Center,Xijing Hospital,Air Force Medical University,Xi'an 710032;Department of Chinese Materia Medica and Natural Medicines,School of Pharmacy,Air Force Medical University,Xi'an 710032;College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang Shaanxi 712046)

机构地区：[1]空军军医大学第一附属医院采购中心,西安710032 [2]空军军医大学药学系中药与天然药物学教研室,西安710032 [3]陕西中医药大学药学院,陕西咸阳712046

出　　处：《中南药学》2021年第11期2263-2268,共6页Central South Pharmacy

基　　金：陕西省科技统筹创新工程计划项目(No.2013KTCL03-10);陕西省重点研发计划(No.2020SF-311)。

摘　　要：目的研究红毛七的皂苷类化学成分。方法采用硅胶柱色谱、反相ODS柱色谱、Sephadex LH-20凝胶柱色谱和半制备高效液相色谱等方法,从红毛七70%乙醇提取物的正丁醇萃取部位分离化合物,通过波谱分析和化学手段鉴定化学结构。结果分离得到5个三萜皂苷类成分,分别鉴定为3-O-β-D-吡喃葡萄糖-(1→2)-α-L-吡喃阿拉伯糖-白桦酸-28-O-α-L-吡喃鼠李糖-(1→4)-β-D-吡喃葡萄糖-(1→6)-β-D-吡喃葡萄糖苷(1)、HN-saponin F(2)、红毛七皂苷D(3)、3-O-β-D-吡喃葡萄糖-(1→2)-α-L-吡喃阿拉伯糖-齐墩果酸-28-O-α-L-吡喃鼠李糖-(1→4)-β-D-吡喃葡萄糖-(1→6)-β-D-吡喃葡萄糖苷(4)和arboreaside D(5)。结论化合物1为新化合物,且首次在红毛七属植物中发现羽扇豆烷型三萜皂苷,化合物5为首次从该属植物中分离得到。Objective To determine the saponin constituents from the roots and rhizomes of Caulophyllum robustum.Methods The compounds from the n-butanol fraction after 70%ethanol extraction were isolated and purified by column chromatographies of silica gel,ODS,Sephadex LH-20 and semi-preparative HPLC.Their structures were identified by spectroscopic analysis and chemical methods.Results Five triterpenoid saponins were isolated and identified as 3-O-α-L-glucopyranosyl-(1→2)-α-L-arabinopyranosyl-betulinic acid-28-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester(1),HN-saponin F(2),auloside D(3),3-O-β-D-glucopyranosyl-(1→2)-α-L-arabinopyranosyl-oleanolic acid-28-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester(4),and arboreaside D(5),respectively.Conclusion Compound 1 is a new compound and this lupane-type triterpenoid saponin has been firstly found from genus Caulophyllum.Compound 5 was obtained from the genus for the first time.

关 键 词：红毛七 三萜皂苷 羽扇豆烷型 白桦酸 

分 类 号：R284[医药卫生—中药学]