半夏SSR分子标记开发与遗传多样性  

Development of SSR molecular markers and genetic diversity analysis in Pinellia ternata(Thunb.)Breit.

作　　者：张景 李晓东 宗庆波 何贝贝 拉基 景纳良 王柯月 钟淑梅 舒少华[1] ZHANG Jing;LI Xiaodong;ZONG Qingbo;HE Beibei;LA Ji;JING Naliang;WANG Keyue;ZHONG Shumei;SHU Shaohua(College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Hubei Province Fruit Tea Office,Wuhan 430070,China;Jiuxin Institute of Traditional Chinese Medicine,Jiuzhoutong Pharmaceutical Group,Wuhan 430051,China)

机构地区：[1]华中农业大学植物科学技术学院,武汉430070 [2]湖北省果茶办公室,武汉430070 [3]九州通医药集团九信中药研究院,武汉430051

出　　处：《华中农业大学学报》2021年第6期19-26,共8页Journal of Huazhong Agricultural University

基　　金：国家重点研发计划项目“中药材生态种植技术研究及应用”(2017YFC1700700);湖北省技术创新重大项目“湖北省重点道地药材品种选育及配套关键技术研究”(2017ACA175);知音英才计划项目“半夏优良品种培育及产业化”;湖北省农业科技创新行动。

摘　　要：对半夏转录组数据进行分析,设计开发SSR引物,利用筛选出的SSR引物分析不同居群间半夏的遗传多样性,为半夏分子标记辅助育种提供支撑。结果显示:筛选出的19对多态性较高的引物在17个半夏居群中共检测到49个多态性位点,具有较高的多态性;利用UPGMA作图共将17个半夏居群分为3个类群;通过分子方差分析(AMOVA)发现半夏居群内的变异达到88%,说明群体与群体间的差异相对较小,遗传变异主要来源于个体间;居群间平均分化系数Fst为0.124,居群间基因流Nm值为1.765,表明居群间能正常地进行基因交流,且居群间遗传分化受基因流的影响较大。本研究利用SSR技术开发的19对SSR引物在不同半夏居群间具有一定的通用性、多态性,能将17份半夏材料明确区分。SSR primers were designed and developed according to unigenes by analyzing the transcriptome data of Pinellia ternate.The screened SSR primers were used to analyze the genetic diversity among different populations of Pinellia ternata and provide support for molecular marker-assisted breeding of Pinellia ternata.The results showed that the selected 19 pairs of primers with high polymorphism detected 49 polymorphic loci in 17 Pinellia populations,indicating that the polymorphism is high.The 17 Pinellia populations were divided into 3 taxa using UPGMA mapping.The analysis of molecular variance(AMOVA)revealed that the variation within the Pinellia population reached 88%,indicating that the difference between populations is relatively small,and the genetic variation mainly comes from between individuals.The average differentiation coefficient Fst between populations was 0.124,and the gene flow Nm value was 1.765,indicating that gene exchange can be carried out normally among populations,and the genetic differentiation among populations is greatly affected by gene flow.The 19 pairs of SSR primers developed by SSR technology in this article have certain versatility and polymorphism among different Pinellia populations,and can clearly distinguish 17 Pinellia materials.

关 键 词：半夏 转录组 简单重复序列分子标记 遗传多样性 居群 

分 类 号：S567.239[农业科学—中草药栽培]