厚朴花浸出物测定方法的建立与不同产地药材质量评价  

Determination Method of Magnoliae Officinalis Flos Extract and Quality Evaluation of Magnoliae Officinalis Flos from Different Producing Areas

作　　者：干丽 洪婉敏 田甜 李燕珍 夏长青 孙冬梅 GAN Li;HONG Wan-min;TIAN Tian;LI Yan-zhen;XIA Chang-qing;SUN Dong-mei(Guangdong Yifang Pharmaceutical Co.,Ltd.,Guangdong Provincial Key Laboratory Traditional Chinese Medicine Formula Granule,Foshan 528244,China)

机构地区：[1]广东一方制药有限公司/广东省中药配方颗粒企业重点实验室,广东佛山528244

出　　处：《中国现代中药》2021年第10期1755-1761,共7页Modern Chinese Medicine

基　　金：广东省省级科技计划项目(2018B030323004)。

摘　　要：目的:建立厚朴花超高效液相色谱法(UPLC)特征图谱与浸出物的测定方法,筛选最佳厚朴花药材产地,为厚朴花药材质量评价提供依据。方法:采用冷浸法与热浸法为提取方法,以水、50%乙醇、75%乙醇、95%乙醇为提取溶剂提取厚朴花浸出物,测定浸出物含量。采用WatersACQUITYUPLCHSST3色谱柱(100 mm×2.1 mm,1.8μm);流动相为乙腈-0.1%甲酸,梯度洗脱;柱温30℃;流速0.35 mL·min^(-1);检测波长300 nm,测定厚朴酚与和厚朴酚含量。以厚朴花浸出物含量、厚朴酚与和厚朴酚总含量、特征图谱共有峰总峰面积作为综合评价指标,优选厚朴花浸出物的测定方法,并通过测定不同产地厚朴花浸出物与特征图谱,评价不同产地厚朴花药材的质量。结果:建立了厚朴花药材的UPLC特征图谱共有模式,标定了12个共有峰,不同产地10批药材总峰面积为13.18~43.66,均值为24.21。建立了厚朴花药材的含量测定方法,不同产地10批药材厚朴酚与和厚朴酚总质量分数为0.31%~0.80%,均值为0.48%;厚朴花浸出物测定方法选择热浸法,75%乙醇为溶剂,不同产地10批药材浸出物质量分数为16.28%~24.36%,均值为20.42%。结论:建立的厚朴花浸出物测定方法稳定、可靠,为厚朴花浸出物限度的制定提供了参考;以四川省产的厚朴花药材质量为佳,对厚朴花药材质量评价及生产利用具有一定的参考意义。Objective:This paper aims to establish the characteristic chromatogram and extraction method for Magnoliae Officinalis Flos and select the optimal producing area for it,which is expected to lay a basis for quality evaluation of the medicinal material.Methods:Magnoliae Officinalis Flos was extracted by hot soaking and room-temperature soaking,respectively,with the solvents of water,50%ethanol,75%ethanol,and 95%ethanol.Ultra performance liquid chromatography(UPLC)conditions are as below:Waters ACQUITY UPLC HSS T3 column(100 mm×2.1 mm,1.8μm),gradient elution with mobile phase of acetonitrile(A)-0.1%formic acid solution(B),flow rate of 0.35 mL·min^(-1),column temperature of 30℃,and detection wavelength of 300 nm.The content of magnolol and honokiol was determined and the total content of the extract,total content of magnolol and honokiol,and the total common peak area of Magnoliae Officinalis Flos were used as comprehensive evaluation indexes to screen the optimal extraction method.The quality of Magnoliae Officinalis Flos from different areas was evaluated based on the extract content and characteristic chromatograms.Results:The UPLC characteristic profile of Magnoliae Officinalis Flos was established,demonstrating 12 common peaks.The total peak area of 10 batches of the medicinal material from different areas was in the range of 13.18-43.66,with the average of 24.21.A method for determining the extract content of Magnoliae Officinalis Flos was thus established.The average total content of magnolol and honokiol in the 10 batches was 0.48%(0.31%-0.80%).Hot soaking with 75%ethanol was selected for the extraction of Magnoliae Officinalis Flos,and the average extract content of the 10 batches of the medicinal material was 20.42%(16.28%-24.36%).Conclusion:The method for determining Magnoliae Officinalis Flos extract established in this study is stable and reliable,which can be used for setting the extract limit of Magnoliae Officinalis Flos.The quality of Magnoliae Officinalis Flos from Sichuan Province is the best,

关 键 词：厚朴花 特征图谱 浸出物 质量评价 

分 类 号：R282.7[医药卫生—中药学]