白藜芦醇对急性肺损伤大鼠的抗炎作用研究  

The anti-inflammatory effects study of resveratrol on acute lung injury rats

作　　者：朱诗乒[1] 楼雅芳[1] 黄小芳[1] 丁旭春[1] 胡晶晶 邓浩然 蔡宛如 ZHU Shiping;LOU Yafang;HUANG Xiaofang;DING Xuchun;HU Jingjing;DENG Haoran;CAI Wanru(Department of Respiratory Medicine,Hangzhou Hospital of Traditional Chinese Medicine,Hangzhou 310007,China;Department of Respiratory Medicine,the Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310005,China)

机构地区：[1]杭州市中医院呼吸科,浙江杭州310007 [2]浙江中医药大学附属第二医院呼吸科,浙江杭州310005

出　　处：《中国现代医生》2021年第27期34-36,40,F0003,共5页China Modern Doctor

基　　金：国家自然科学基金委员会资助项目(81774220);浙江省杭州市卫生计生科技计划项目(2018A67)。

摘　　要：目的通过动物实验验证中药虎杖主要成分白藜芦醇对急性肺损伤大鼠的抗炎保护作用。方法40只SD大鼠随机分为空白对照组、肺损伤模型组、溶剂对照组和白藜芦醇治疗组,每组各10只。气道内滴注细菌内毒素脂多糖(LPS)制备急性肺损伤模型,溶剂对照组腹腔注射单体溶解剂(脂肪乳),白藜芦醇治疗组腹腔注射白藜芦醇及溶解剂,分别在造模前24 h、造模时及造模后24 h内进行腹腔内注射。造模后48 h进行标本采集,肺组织进行病理染色检查并检测肺泡灌洗液(BALF)中性粒细胞比值、肿瘤坏死因子-α(TNF-α)及乳酸脱氢酶(LDH)的含量。结果白藜芦醇治疗组的肺泡灌洗液中性粒细胞比值及TNF-α数值为(68.432±20.623)、(92.644±11.322)ng/L,高于溶剂对照组的(87.023±4.032)、(106.654±4.606)ng/L,差异有统计学意义(P<0.05);两组肺泡灌洗液中LDH数值比较,差异无统计学意义(P>0.05)。结论白藜芦醇治疗组能显著减轻急性肺损伤引起的病理改变,白藜芦醇对急性肺损伤大鼠具有抗炎保护作用。Objective To evaluate the protective efficacy of resveratrol,an effective component of polygonum cuspidatum,on acute lung injury through animal experiments.Methods A total of 40 SD rats were randomly divided into the blank group(n=10),the model group(n=10),the solvent control group(n=10)and the resveratrol treatment group(n=10).The acute lung injury model was established by intratracheal instillation of lipopolysaccharide(LPS).The solvent group was intraperitoneally injected with monomer dissolvent agent(fat emulsion),while the resveratrol treatment group was intraperitoneally injected with resveratrol and solubilizer.They were injected intraperitoneally 24 hours before,during and within 24 hours after the model was established.Samples were collected 48 hours after the model was established.Meanwhile,the lung tissues were examined by pathological staining,and the neutrophil ratio,tumor necrosis factor-α(TNF-α)and lactate dehydrogenase(LDH)contents in bronchoalveolar lavage fluid(BALF)were detected.Results The neutrophil ratio and TNF-αin BALF of resveratrol treatment group were(68.432±20.623),(92.644±11.322)ng/L,which were higher than those of(87.023±4.032),(106.654±4.606)ng/L in the solvent control group,with statistically significant differences(P<0.05).There was no statistically significant difference in LDH values between the two groups of BALF(P>0.05).Conclusion Resveratrol treatment group can significantly reduce pulmonary interstitial inflammatory cell infiltration,pulmonary interstitial edema,bronchoalveolar wall thickening and bronchoalveolar cavity destruction degree.Resveratrol has certain anti-inflammatory efficacy on rats with acute lung injury.

关 键 词：急性肺损伤 大鼠 白藜芦醇 肿瘤坏死因子-Α 

分 类 号：R331[医药卫生—人体生理学]