ORFV诱导HaCaT细胞CK1等分子mRNA转录波动  

ORFV Induced CK1 and Other Molecular mRNA Transcriptional Fluctuation in HaCaT Cells

作　　者：刘圆圆 张帆 于永忠[1] Liu Yuanyuan;Zhang Fan;Yu Yongzhong(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2021年第5期86-92,共7页journal of heilongjiang bayi agricultural university

基　　金：黑龙江八一农垦大学研究生创新科研项目(荷兰马齿苋不饱和脂肪酸抗ORFV感染的分子机制:YJSCX2019-Y67);黑龙江八一农垦大学学位与研究生教育教学改革研究项目(基于“发展式”理念的生物学研究生双创能力培养机制构建:YJG201906)。

摘　　要：羊传染性脓疱病毒(orf virus,ORFV)可引起动物口唇等黏膜上皮异常角化,导致严重的增生性病变。由于临床存在复杂的患病风险,使ORFV感染早期对症治疗至关重要。因此,研究病毒对细胞增殖与分化的影响有助于筛选抗病毒路径。研究表明,角质形成细胞的增殖和分化进程直接受到角蛋白(CKs)等分子转换调控。为研究ORFV感染对CKs转换的影响,试验室利用ORFV-HaCaT细胞体外感染模型,通过RT-PCR方法检测病毒感染的不同时间段DSG3、Ki67和CKs转录水平。结果显示,DSG3、Ki67、CK2分子表达一直处于稳定,而CK1/CK10、CK5/CK14则在ORFV感染早期相继发生短暂的正向波动,但很快又受到病毒抑制,表明病毒会持续调节感染诱导影响细胞分化趋势。研究为深入探讨ORFV引起角质细胞CKs波动的分子机制奠定了基础。Orf virus(ORFV)can cause abnormal keratosis of the mucosal epithelium where on the mouth and lip of animals,leading to severe hyperplastic lesions.Because of the complex clinical risk,early symptomatic treatment of ORFV infection was very important.Therefore,studying the effect of virus on cell proliferation and differentiation was helpful to screen antiviral pathways.Studies had shown that the proliferation and differentiation processes of keratinocytes were directly regulated by molecular transformations such as keratin(CKs).To investigate the effect of ORFV infection on CKs conversion,ORFV-HaCaT cell infection model in vitro was used to detect the transcription levels of DSG3、Ki67 and CKS at different time periods of viral infection by RT-PCR.Results showed that DSG3、Ki67、CK2 molecular expression had been in stable,whereas CK1/CK10 and CK5/CK14 occurred in the early infection of ORFV brief positive fluctuation,but quickly by virus inhibition,suggesting that the virus will continue to adjust the infection induced by affecting cell differentiation trend.It provided a basis for further study of the molecular mechanism of keratinocyte CKS fluctuations induced by ORFV.

关 键 词：羊口疮病毒感染 HACAT细胞 角蛋白分子 表达变化 RT-PCR 

分 类 号：R751[医药卫生—皮肤病学与性病学]