洋甘菊总黄酮对高脂血症模型小鼠脂质代谢的影响及机制  

Effects of Total Flavonoids from Chamomile on Lipid Metabolism of Hyperlipidemia Model Mice and Its Mechanism

作　　者：兰卫[1] LAN Wei(College of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830017,China)

机构地区：[1]新疆医科大学中医学院,乌鲁木齐830017

出　　处：《中国药房》2021年第22期2706-2712,共7页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81960771);国家重点研发计划项目(No.2017YFC1703902-3);新疆维吾尔自治区自然科学基金资助项目(No.2018D01C160)。

摘　　要：目的:研究洋甘菊总黄酮对高脂血症模型小鼠脂质代谢的影响及潜在机制。方法:将30只雄性载脂蛋白E基因缺陷(C57BL/6J-ApoE^(-/-))小鼠随机分成模型组、阳性对照组(非诺贝特30 mg/kg)和洋甘菊总黄酮低、中、高剂量组(88、176、352 mg/kg),每组6只;另取6只雄性C57BL/6J小鼠作为正常对照组。正常对照组小鼠用普通饲料喂养,其余各组小鼠均用高脂饲料喂养8周以复制高脂血症模型。造模同时,各给药组小鼠灌胃相应药液(均以1%羧甲基纤维素钠溶液为溶剂),正常对照组和模型组小鼠灌胃1%羧甲基纤维素钠溶液,每次灌胃200μL,每天1次,连续8周。分别于给药前和给药8周后称定各组小鼠的体质量,测定末次给药后小鼠血清中总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)含量,肝组织中超氧化物歧化酶(SOD)、丙二醛(MDA)含量以及过氧化物酶体增殖物激活受体α(PPARα)、肉毒碱棕榈酰基转移酶1A(CPT1A)、过氧化物酶酰基辅酶A氧化酶1(ACOX1)蛋白的表达水平,并观察肝组织的病理改变。结果:与给药前比较,各组小鼠给药8周后的体质量均有升高趋势。与正常对照组比较,模型组小鼠给药8周后的体质量和血清中TC、TG、LDL-C、AST、ALT含量以及肝组织中MDA含量均显著升高(P<0.05或P<0.01),血清中HDL-C含量和肝组织中SOD含量以及PPARα、CPT1A、ACOX1蛋白的表达水平均显著降低(P<0.05或P<0.01),肝组织结构紊乱并可见大小不一的圆形脂肪空泡,细胞质中可见大小不等的脂滴。与模型组比较,洋甘菊总黄酮各剂量组和阳性对照组小鼠给药8周后的体质量(除洋甘菊总黄酮低剂量组外)和血清中TC、TG、LDL-C、AST、ALT含量以及肝组织中MDA(除洋甘菊总黄酮低、中剂量组外)含量均显著降低(P<0.05或P<0.01),血清中HDL-C含量和肝组织中SOD含量以及OBJECTIVE:To study the effects of total flavonoids from chamomile on lipid metabolism of hyperlipidemia model mice and its potential mechanism.METHODS:Thirty male C57BL/6J-ApoE^(-/-)mice were randomly divided into model group,positive control group(fenofibrate 30 mg/kg)and chamomile total flavonoids low-dose,medium-dose and high-dose groups(88,176,352 mg/kg),with 6 mice in each group.In addition,6 male C57BL/6J mice were used as normal control group.Mice in normal control group were fed with ordinary diet,and mice in other groups were fed with high-fat diet for 8 weeks to replicate hyperlipidemia model.At the time of making model,administration groups were given relevant liquid(using 1%sodium carboxymethyl cellulose as solvent);normal control group and model group were given 1%sodium carboxymethyl cellulose intragastrically,200 mL per gavage,once a day,for consecutive 8 weeks.The body weight of mice in each group was weighed before medication and 8 weeks after medication.The serum contents of total cholesterol(TC),triacylglycerol(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in mice were detected after last administration;the contents of superoxide dismutase(SOD)and malondialdehyde(MDA)as well as the protein expressions of peroxisome proliferator-activated receptorα(PPARα),carnitine palmityl transferase 1A(CPT1A)and peroxase acyl-CoA oxidase 1(ACOX1)in liver tissue were determined.The pathological changes in liver tissue were observed.RESULTS:Compared with before medication,the body weight of each group showed an increasing trend after 8 weeks of medication.Compared with normal control group,body weight,the contents of TC,TG,LDL-C,AST and ALT in serum and MDA content in liver tissue of mice in model group were significantly increased after 8 weeks of medication(P<0.05 or P<0.01).The content of HDL-C in serum and the content of SOD in liver tissue,as well as the protein expressions of PPARα,CPT1A and ACO

关 键 词：洋甘菊总黄酮 高脂血症 载脂蛋白E基因缺陷小鼠 脂质代谢 作用机制 

分 类 号：R965[医药卫生—药理学]