UHPLC法结合超滤法测定连钱草中3种成分的血浆蛋白结合率  

Determination of Plasma Protein Binding Rate of 3 Components from Glechoma longituba by UHPLC Combined with Ultrafiltration

作　　者：陈伟康 刘德鸿 熊明朋 袁惠 周国平 CHEN Weikang;LIU Dehong;XIONG Mingpeng;YUAN Hui;ZHOU Guoping(Jiangxi Institute for Drug Control/NMPA Key Laboratory of Quality Evaluation of Chinese Patent Medicine/Jiangxi Province Engineering Research Center of Drug and Medical Device Quality,Nanchang 330029,China)

机构地区：[1]江西省药品检验检测研究院/国家药品监督管理局中成药质量评价重点实验室/江西省药品医疗器械质量工程研究中心,南昌330029

出　　处：《中国药房》2021年第22期2720-2723,共4页China Pharmacy

基　　金：江西省重点研发计划项目(No.20171BBG70104)。

摘　　要：目的:建立测定连钱草中迷迭香酸、咖啡酸、绿原酸血浆蛋白结合率的方法。方法:采用超高效液相色谱法结合超滤法测定连钱草中迷迭香酸、咖啡酸、绿原酸在新西兰兔体内的血浆蛋白结合率。以Phenomenex Luna^(■)C_(18)为色谱柱,以乙腈(A)-0.1%甲酸溶液(B)为流动相进行梯度洗脱,流速为0.5 mL/min,柱温为45℃,检测波长为327 nm,进样量为3μL。结果:在低、中、高质量浓度下,迷迭香酸的血浆蛋白结合率分别为(97.78±1.67)%、(94.32±1.42)%、(95.12±1.51)%(n=3),咖啡酸的血浆蛋白结合率分别为(90.12±2.33)%、(89.53±1.98)%、(90.23±1.56)%(n=3),绿原酸的血浆蛋白结合率分别为(63.23±2.12)%、(67.87±1.06)%、(62.34±1.34)%(n=3)。结论:所建方法操作简单、分析时间较短,可用于测定连钱草中迷迭香酸、咖啡酸、绿原酸的血浆蛋白结合率。OBJECTIVE:To establish a method for the determination of plasma protein binding rate of rosmarinic acid,caffeic acid and chlorogenic acid from Glechoma longituba.METHODS:UHPLC method combined with ultrafiltration method was adopted to determine the plasma protein binding rate of rosmarinic acid,caffeic acid and chlorogenic acid from G.longituba in the plasma of New Zealand rabbits.The determination was performed on a Phenomenex Luna®C_(18) column with mobile phase consisted of acetonitrile(A)-0.1%formic acid solution(B)(gradient elution)at the flow rate of 0.5 mL/min.The column temperature was set at 45℃,and the detection wavelength was 327 nm.The sample size was 3μL.RESULTS:At low,medium and high concentrations,the plasma binding rates of rosmarinic acid were(97.78±1.67)%,(94.32±1.42)%,(95.12±1.51)%,respectively(n=3);those of caffeic acid were(90.12±2.33)%,(89.53±1.98)%,(90.23±1.56)%,respectively(n=3);those of chlorogenic acid were(63.23±2.12)%,(67.87±1.06)%,(62.34±1.34)%,respectively(n=3).CONCLUSIONS:Established method is easy to operate and shorter time for analysis.It can be used to determine the plasma protein binding rate of rosmarinic acid,caffeic acid and chlorogenic acid in G.longituba.

关 键 词：连钱草 迷迭香酸 咖啡酸 绿原酸 血浆蛋白结合率 超高效液相色谱 超滤法 兔 

分 类 号：R969.1[医药卫生—药理学] R927.2[医药卫生—药学]