加载PEDF的多聚体超声微泡研制及其特性  

Preparation of PEDF-loaded polymer ultrasound microbubbles and its properties

作　　者：胡劼[1,2] 焦明克 刘立洁[3] 张鹏[3] 牛盈盈[3] HU Jie;JIAO Mingke;LIU Lijie;ZHANG Peng;NIU Yingying(Department of Echocardiography,the Fourth Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China;Department of Ultrasound,Yubei District People's Hospital of Chongqing,Chongqing 400000,China;Department of Biomedical Engineering,General Hospital of Xinjiang Military Region,Urumqi 830000,China)

机构地区：[1]新疆医科大学第四附属医院心脏超声科,新疆乌鲁木齐830000 [2]重庆市渝北区人民医院超声科,重庆400000 [3]新疆军区总医院医学工程科,新疆乌鲁木齐830000

出　　处：《中国医学物理学杂志》2021年第11期1417-1420,共4页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(81760313)。

摘　　要：目的:针对血管新生类疾病,研制与色素上皮源因子(PEDF)具有最大结合率的多聚体超声微泡,并明确其理化特性。方法:首先应用声空化方法制备多聚体超声微泡;然后将PEDF按浓度分为0.4、2.0、10.0和50.0μg/mL组,应用跨膜按梯度法包裹入脂质体,使其与超声微泡结合,进而置于荧光显微镜下观察。应用流式细胞仪检测PEDF与超声微泡的最大结合率,采用荧光分光光度法测定载药超声微泡的包封率及体外释药特性。结果:成功制备了与PEDF最大结合率为(96.14±1.21)%的多聚体超声微泡。脂质体对PEDF的包封率约为(79.20±2.31)%,体外释放浓度于微泡击破后1 min内达到峰值,持续释放10 min后浓度显著减低。结论:与PEDF具有较高结合率的多聚体超声微泡可以作为合适的药物载体,靶向治疗血管新生类疾病。Objective To develop the polymer ultrasound microbubbles with the maximum binding rate to pigm entepithelium-derived factor(PEDF)for angiogenic diseases,and to clarify the physical and chemical properties.Methods Polymer ultrasound microbubbles were prepared by acoustic cavitation method.After PEDF was divided into different groups(0.4,2.0,10.0 and 50.0μg/mL)according to the concentration,it was encapsulated with liposomes using transmembrane gradient method,which enabled PEDF to bind to ultrasound microbubbles,and then the binding was observed under fluorescence microscope.The maximum binding rate of PEDF to ultrasound microbubbles was obtained by flow cytometry.Moreover,the efficiency of encapsulating PEDF into liposomes and the in vitro drug release characteristics of the drug-loaded ultrasound microbubbles were determined by fluorescence spectrophotometry.Results The polymer ultrasound microbubbles which had a maximum binding rate of(96.14±1.21)%to PEDF was successfully prepared.The efficiency of encapsulating PEDF into liposomes was about(79.20±2.31)%.The in vitro drug release concentration reached the maximum in 1 min after the microbubble breaking,and the concentration was obviously reduced after the continuous release for 10 min.Conclusion The polymer ultrasound microbubbles with a high binding rate to PEDF can be used as a suitable drug loader for targeted treatment of angiogenic diseases.

关 键 词：血管新生类疾病 色素上皮源因子 超声微泡 靶向治疗 

分 类 号：R318[医药卫生—生物医学工程]