微生物法测定特殊医学用途配方食品中生物素含量的不确定度评定  

Uncertainty Evaluation for Microbiological Determination of Biotin in Food for Special Medical Use

作　　者：孟静 吴裕健 钟立霞 程祥龙 王锐 MENG Jing;WU Yu-jian;ZHONG Li-xia;CHENG Xiang-long;WANG Rui(Shandong Institute for Food and Drug Control,Jinan,Shandong 250100,China;College of Chemical and Pharmaceutical Engineering,Shandong First Medical University,Taian,Shandong 271016,China)

机构地区：[1]山东省食品药品检验研究院,山东济南250100 [2]山东第一医科大学化学与制药工程学院,山东泰安271016

出　　处：《计量学报》2021年第10期1404-1408,共5页Acta Metrologica Sinica

摘　　要：特殊医学用途配方食品是部分人群摄取营养的唯一来源,其中生物素的准确定量具有重要的意义。采用GB 5009.259—2016中规定的微生物法进行生物素含量测定,并评定了标准不确定度和扩展不确定度,测定结果生物素含量为(27.16±1.15)μg/100 g,p=95%,k=2。分析了各个分量对不确定度的贡献,结果表明:实验重复性为主要影响因素,为微生物法测定生物素的关键控制点。The formula food for special medical use is the only source of nutrition for some people,and the accurate quantification of biotin is of great significance. The microbiological method specified in GB 5009. 259—2016 was used to determine the content of biotin. The standard uncertainty and expanded uncertainty of biotin were evaluated. The results showed that the content of biotin was( 27. 16 ± 1. 15) μg/100 g,p = 95%,k = 2. The contribution of each component to the uncertainty was analyzed. The results showed that the experimental repeatability was the main influencing factor and the key control point for the determination of biotin by microbiological method.

关 键 词：计量学 生物素 配方食品 微生物法 不确定度 

分 类 号：TB99[一般工业技术—计量学]