冠心病血瘀证“瘀毒”病机转变的蛋白质组学研究  

Proteomic Study on Pathogenesis of"Blood-stasis and Toxin"in Patients with Coronary Heart Disease and Blood Stasis Syndrome

作　　者：尚青华[1] 徐浩[1] 史大卓[1] 陈可冀[1] SHANG Qinghua;XU Hao;SHI Dazhuo;CHEN Keji(Xiyuan Hospital of Chinese Academy of Traditional Chinese Medicine,Beijing 100091,China)

机构地区：[1]中国中医科学院西苑医院,北京100091

出　　处：《中西医结合心脑血管病杂志》2021年第22期3825-3829,共5页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然科学基金项目(No.30973702);973计划专项课题(No.2006CB504803);中国中医科学院自主选题研究项目(No.ZZ0908051)。

摘　　要：目的探索冠心病血瘀证发展过程中“因瘀致毒”病机转变的分子标志物。方法纳入1269例稳定期冠心病血瘀证病人并留存血样,随访1年,随访期间发生急性心血管事件病人血样设为A组,匹配随访未发生急性心血管事件病人血样为B组,随访半年以后发生急性心血管事件且血清保存完好的病人血样设为C组,另选取急性心肌梗死病人血样设为D组。采用Maldi-Tof-MS法对不同人群的差异蛋白进行比较分析和鉴定。结果比较4组的差异蛋白,利用1945.31 Da、1981.25 Da进行建模,得到事件组的识别率为90.2%,预测率为89.6%。质谱鉴定这两个蛋白分别是同种型高分子量激肽原1的前体(KNG1)、过氧化物还原酶-1(PRDX1)。结论KNG1和PRDX1可考虑作为冠心病“因瘀致毒”病机转变的分子标志物,值得进一步验证。Objective To find the sensitive biomarkers of pathogenesis transformation in the progress of"blood-stasis to toxin syndrome".Methods A 1-year follow-up was performed in 1269 coronary heart disease and blood stasis syndrome(CHD-BSS)patients,acute cardiovascular events(ACEs)were recorded.Patients who undergone ACEs in 1 year follow-up period were defined as"potential toxin syndrome"group(group A),and patients without ACEs were defined as control group(group B).The patients undergone ACEs between 0.5 to 1 year were defined as group C.The patients attacked with acute myocardial infarction were defined as"toxin syndrome"group(group D).Maldi-Tof-MS technology was used to analyze and identify the differential proteins in these groups.Results Two peptides(1945.31 Da and 1981.25 Da)which distinguished from 4 groups were made for a model,the discrimination power of this model for ACEs was 90.2%,and the predictive values was 89.6%.The peptide of 1945.31 Da was isoform HMW of kininogen-1 precursor(KNG1),and the peptide of 1981.25 Da was peroxiredoxin-1(PRDX1)identified by mass spectrometry.Conclusion KNG1 and PRDX1 might be identified as the biomarkers of pathogenesis transformation(blood-stasis to toxin),and worhty of further verified.

关 键 词：冠心病 血瘀证 瘀毒 毒证 蛋白质组 

分 类 号：R54[医药卫生—心血管疾病]