葛根总黄酮干预长时耐力运动后再力竭运动大鼠脑组织β-连环蛋白及糖原合成酶激酶3β的表达  

Effects of Pueraria lobata flavonoids on expressions of beta-catenin and glycogen synthase kinase 3beta in the brain of rats undergoing exhaustive exercise after long endurance exercise

作　　者：莫伟彬[1,2] 黄天昌 曾智伟[1] 燕林博 Mo Weibin;Huang Tianchang;Zeng Zhiwei;Yan Linbo(College of Physical Education and Health,Guangxi Normal University,Guilin 541004,Guangxi Zhuang Autonomous Region,China;State Key Laboratory of Medicinal Resources Chemistry and Molecular Engineering,Guilin 541004,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]广西师范大学体育与健康学院,广西壮族自治区桂林市541004 [2]药用资源化学与药物分子工程国家重点实验室,广西壮族自治区桂林市541004

出　　处：《中国组织工程研究》2022年第5期736-741,共6页Chinese Journal of Tissue Engineering Research

基　　金：广西教育厅科研项目(2021KY1596),项目参与人:莫伟彬;广西教育厅科研项目(2018KY0090),项目负责人:莫伟彬;教育发展基金会“教师成长基金”项目(EDF2016005),项目负责人:莫伟彬;国家重点实验室开放项目(CMEMR2012-B04),项目负责人:莫伟彬。

摘　　要：背景:长时间的力竭运动易引起大鼠缺血缺氧,致使脑组织损伤,葛根总黄酮具有保护大鼠心脑血管、改善脑损伤神经和抗氧化等作用,但运动前补充葛根总黄酮是否达到保护脑组织作用目前尚不明确。目的:观察葛根总黄酮对运动大鼠脑组织病理改变和β-连环蛋白及糖原合成酶激酶3β表达的影响。方法:将50只SD雄性大鼠随机分为安静对照组、运动对照组、葛根总黄酮低、中、高剂量组。除安静对照组外,其余各组大鼠进行为期6周的运动训练,于6周末即最后一次训练达到力竭。葛根总黄酮低、中、高剂量组大鼠均在运动前20 min灌胃50,100,200 mg/kg的葛根总黄酮,1次/d,直到实验结束。苏木精-伊红染色观察脑组织病理学变化,免疫组织化学法、RT-PCR法及Western blot法测定β-连环蛋白和糖原合成酶激酶3β表达。结果与结论:(1)光镜观察运动对照组大鼠脑皮质和海马区有部分神经细胞胞体肿胀、胞体收缩、脑膜或室管膜显现水肿、充血和出血等现象,葛根总黄酮低剂量组有水肿、充血和出血等轻微症状,而中、高剂量组大鼠脑组织病理变化与安静对照组比较无明显差异;(2)免疫组织化学染色显示,运动对照组大鼠脑组织β-连环蛋白和糖原合成酶激酶3β表达均高于安静对照组(P <0.01),葛根总黄酮中、高剂量组大鼠脑组织β-连环蛋白和糖原合成酶激酶3β表达均低于运动对照组(P <0.01);(3)运动对照组大鼠脑组织β-连环蛋白、糖原合成酶激酶3βmRNA及蛋白表达均高于安静对照组(P <0.05或P <0.01);葛根总黄酮中、高剂量组大鼠脑组织β-连环蛋白、糖原合成酶激酶3βmRNA及蛋白表达均低于运动对照组(P <0.05或P <0.01);(4)结果表明,力竭运动引起大鼠脑组织损伤和β-连环蛋白、糖原合成酶激酶3β表达上调,葛根总黄酮可能是通过下调β-连环蛋白和糖原合成酶激酶3β的表达进而有效改善大�BACKGROUND: Prolonged exhaustive exercise can easily cause ischemia and hypoxia in rats, leading to brain tissue damage. Pueraria lobata flavonoids can exert a series of roles, including cardiovascular and cerebrovascular protections, improvement of brain damage and anti-oxidation. However, whether supplementation of Pueraria lobata flavonoids prior to exercise can protect the brain tissue is still unclear.OBJECTIVE: To observe the effect of total flavonoids in Pueraria lobata on histopathological changes and expressions of β-catenin and glycogen synthase kinase 3β in rats undergoing exercises.METHODS: Fifty Sprague-Dawley male rats were randomly divided into five groups: a quiet control group, an exercise control group, and low-, medium-and high-dose groups of total flavonoids in Pueraria lobata. Except the quiet control group, the remaining groups were trained for 6 weeks, while the last training reached exhaustion on the 6 th weekend. In the low-, middle-and high-dose groups, all the rats were given 50, 100, 200 mg/kg total flavonoids in Pueraria lobata by gavage at 20 minutes before the experiment once a day until the end of the experiment. In all the groups, hematoxylin-eosin staining was used to observe the histopathological changes of the brain, and immunohistochemistry, PCR and western blot were used to determine the expressions of β-catenin and glycogen synthase kinase 3β. RESULTS AND CONCLUSION: Under the light microscope, in the exercise control group, some nerve cells in the cerebral cortex and hippocampus became swollen and contracted, and there were edema, hyperemia and bleeding in the meninges or ependymal membranes. The low-dose group appeared to have some mild symptoms, such as edema, congestion and hemorrhage. However, the pathological changes in the brain tissue of rats in the middle-and high-dose groups were not significantly different from those in the quiet control group. Immunohistochemical staining showed that the expression levels of β-catenin and glycogen synthase kinase 3β in the

关 键 词：葛根总黄酮 力竭运动 脑组织 Β-连环蛋白 糖原合成酶激酶3Β 通路 大鼠 

分 类 号：R455[医药卫生—运动医学] R496[医药卫生—治疗学]