一次性力竭运动模型促进大鼠肾脏NOD样受体蛋白3炎性小体的表达  

A single bout of exhaustive exercise induces renal NOD-like receptor protein 3 inflammasome expression in rats

作　　者：耿元文[1] 林琴琴[1] 李若明 唐韶慨 王柏慧 田振军[2] Geng Yuanwen;Lin Qinqin;Li Ruoming;Tang Shaokai;Wang Baihui;Tian Zhenjun(School of Physical Education,Yanshan University,Qinhuangdao 066004,Hebei Province,China;School of Physical Education,Shaanxi Normal University,Xi′an 710062,Shaanxi Province,China)

机构地区：[1]燕山大学体育学院,河北省秦皇岛市066004 [2]陕西师范大学体育学院,陕西省西安市710062

出　　处：《中国组织工程研究》2022年第2期190-196,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(31300978),项目负责人:林琴琴;河北省自然科学基金(C2019203537),项目负责人:林琴琴;河北省高等学校科学技术研究项目(QN2019068),项目负责人:林琴琴。

摘　　要：背景:力竭运动导致机体多次血氧重新分配,诱发强烈炎症反应,增强全身炎症水平,损伤肾脏功能。目的:基于miR-155/SIRT1/TXNIP通路途径下,探讨一次性力竭运动造成大鼠肾脏炎症损伤的作用及机制。方法:将20只3月龄雄性SD大鼠随机分为对照组和力竭运动组,每组10只,力竭运动组采用三级递增运动负荷跑台训练建立一次性力竭运动实验动物模型,对照组不建模。训练结束后即刻取材,检测血清尿素氮、肌酐、肌酸激酶、丙二醛、胱抑素C和尿液肾损伤分子1水平,检测肾脏硫氧还蛋白结合蛋白水平,采用Western Blot法检测肾脏组蛋白去乙酰化酶SIRT1、NOD样受体蛋白3、凋亡相关斑点样蛋白1、Caspase-1和白细胞介素1β蛋白表达,采用RT-qPCR法检测血清和肾脏miR-155及肾脏SIRT1 mRNA表达,同时进行肾脏细胞形态学观察。结果与结论:(1)与对照组比较,力竭运动组大鼠血清和肾脏miR-155 mRNA表达均增多(P<0.01,P<0.05);(2)与对照组比较,力竭运动组大鼠肾脏SIRT1 mRNA和蛋白表达下降(P<0.01),肾脏硫氧还蛋白结合蛋白水平升高(P<0.01);(3)与对照组比较,力竭运动组大鼠肾脏NOD样受体蛋白3、凋亡相关斑点样蛋白1、Caspase-1和白细胞介素1β蛋白表达均增加(P<0.05或P<0.01),血清尿素氮、肌酐、肌酸激酶、丙二醛、胱抑素C和尿液肾损伤分子1均升高(P<0.05或P<0.01),肾组织病理损伤严重;(4)肾脏SIRT1蛋白表达与硫氧还蛋白结合蛋白、NOD样受体蛋白3蛋白表达呈负相关(r=-0.962,P<0.01;r=-0.977,P<0.01);肾脏miR-155表达与SIRT1蛋白表达呈负相关(r=-0.989,P<0.01),与硫氧还蛋白结合蛋白和NOD样受体蛋白3蛋白表达呈正相关(r=0.902,P<0.01;r=0.968,P<0.05);(5)提示一次性力竭运动可上调miR-155表达、抑制SIRT1表达、增加硫氧还蛋白结合蛋白表达、激活NOD样受体蛋白3炎性小体,引发炎症反应,加重肾组织的病理损伤,损伤肾功能,这可能是力竭运动�BACKGROUND:Exhaustive exercise leads to multiple redistribution of blood oxygen in the body,induces a strong inflammatory response,enhances the level of systemic inflammation,and damages kidney function.OBJECTIVE:To determine the effects of a single bout of exhaustive exercise on the expressions of renal NOD-like receptor protein 3(NLRP3)inflammasome in rats and possible mechanism.METHODS:Twenty male Sprague-Dawley rats aged 3 months were randomly divided into a control group and an exhaustive exercise group,with 10 rats in each group.In the exhaustive exercise,three-level incremental exercise load treadmill training was performed to establish a one-time exhaustive exercise experimental animal model.No modeling was performed in the control group.Immediately after training,blood sample was collected from each rat,and then the kidney tissue was removed for hematoxylin-eosin staining to observe the morphological changes of renal cells.Renal thioredoxin-interacting protein(TXNIP),serum blood urea nitrogen,creatinine,creatine kinase,malondialdehyde,cystatin C and urine kidney injury molecule-1 levels were detected.Western blot was used to detect renal SIRT1,NLRP3,apoptosis-associated speck-like protein containing a CARD(ASC)1,Caspase-1 and interleukin-1βprotein expression.RTqPCR was performed to detect serum and renal miR-155 and renal SIRT1 mRNA expression.Meanwhile,renal cell morphology was observed.RESULTS AND CONCLUSION:Compared with the control group,the levels of serum and renal miR-155 were significantly increased in the exhaustive exercise group(P<0.01,P<0.05).Compared with the control group,the protein and mRNA expression of renal SIRT1 was significantly decreased(both P<0.01),while the protein expression of renal TXNIP,NLRP3,ASC-1,Caspase-1 and interleukin-1βwas significantly increased in the exhaustive exercise group(P<0.05 or 0.01).Moreover,the levels of serum blood urea nitrogen,creatinine,creatine kinase,malondialdehyde,cystatin C and urine kidney injury molecule-1 were significantly increased in the e

关 键 词：一次性力竭运动 肾脏 炎症损伤 MIR-155 SIRT1 NLRP3炎性小体 大鼠 

分 类 号：R459.9[医药卫生—治疗学] R364.5[医药卫生—临床医学]