川续断皂苷Ⅵ修复兔肌腱病  

AsperosaponinⅥtherapy for Achilles tendinopathy in rabbits

作　　者：王坤[1] 何本祥[2] Wang Kun;He Benxiang(Institute of Sports Medicine and Health,Chengdu Sport University,Chengdu 610000,Sichuan Province,China;Affiliated Sports Hospital,Chengdu Sport University,Chengdu 610000,Sichuan Province,China)

机构地区：[1]成都体育学院运动医学与健康研究所,四川省成都市610000 [2]成都体育学院附属体育医院,四川省成都市610000

出　　处：《中国组织工程研究》2022年第2期211-217,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家重点研发计划项目(2019YFF0301704),项目负责人:何本祥。

摘　　要：背景:近年来肌腱病是一种高发的骨科慢性损伤,目前仍缺少有效的治疗手段,川续断具有补肝肾、强筋骨、续折伤等功效。目的:旨在研究川续断中主要成分川续断皂苷Ⅵ对兔肌腱病的修复作用。方法:48只兔随机分为正常组(n=8)、模型组(n=32)和生理盐水组(n=8),其中模型组于左跟骨附着点注射前列腺素E2建立兔跟腱病模型,随后随机分为4个亚组,分别采用0,10,20,40 mg/kg川续断皂苷Ⅵ治疗,1次/d,连续4周。动物实验于2019-11-04经成都体育学院动物伦理委员会批准,批准号:成体伦理[2020]21号。结果与结论:(1)与正常组相比,模型组基质金属蛋白酶1表达水平较高;而与模型组相比,川续断皂苷Ⅵ中、高剂量组基质金属蛋白酶1表达下调(P<0.05);模型组金属蛋白酶抑制剂1水平高于正常组,而在川续断皂苷Ⅵ低、中、高剂量组均可降低金属蛋白酶抑制剂1的表达水平(P<0.05);与模型组相比,川续断皂苷Ⅵ低、中、高剂量转化生长因子β1表达水平上调(P<0.05);与模型组相比,川续断皂苷Ⅵ高剂量纤溶酶原激活物抑制剂1表达水平升高(P<0.05);(2)川续断皂苷Ⅵ高剂量组纤维组织排列及分布恢复均接近正常肌腱组织。提示川续断皂苷Ⅵ可平衡细胞外基质胶原异常代谢,诱导肌腱细胞增殖,利于保持肌腱胶原的稳定性促进愈合,可能是一种潜在的治疗肌腱病的药物。BACKGROUND:In recent years,tendonopathy occurs frequently as a chronic orthopedic injury,and there is still a lack of effective treatment as yet.Dipsacus L.has the effect of tonifying the liver and kidney,strengthening bones,and sustaining broken injuries.OBJECTIVE:To analyze the repairing effect of AsperosaponinⅥin a rabbit model of Achilles tendinopathy.METHODS:Forty-eight rabbits were randomized into a normal control group(n=8),a model group(n=32),and a noral saline group(n=8).In the model group,the model of tendinopathy was made at the left calcaneus attachment injected with prostaglandin E2,and the models were then randomized into four subgroups,which were treated with 0,10,20,and 40 mg/kg AsperosaponinⅥ,respectively.Treatments in each group were conducted once a day for 4 consecutive weeks.The study protocol was approved by the Animal Ethics Committee of Chengdu Sport University with an approval No.[2020]21 on November 4,2019.RESULTS AND CONCLUSION:Compared with the normal control group,the expression of matrix metalloproteinase-1 was significantly higher in the model group(P<0.05).Compared with the model group,the expression of matrix metalloproteinase-1 was significantly reduced in the 20 and 40 mg/kg groups(P<0.05).The expression of tissue inhibitor of matrix metalloproteinases 1 was significantly higher in the model group than the normal control group(P<0.05),while the expression of tissue inhibitor of matrix metalloproteinases 1 was significantly reduced in the 10,20 and 40 mg/kg groups compared with the model group(P<0.05).The expression of transforming growth factor-β1 was significantly higher in the 10,20 and 40 mg/kg groups compared with the model group(P<0.05).The expression of plasminogen activator inhibitor 1 was significantly higher in the 40 mg/kg group than the model group(P<0.05).Tendon fibers in the 40 mg/kg group were intact and arranged orderly,which were similar to normal tendon tissue.Therefore,AsperosaponinⅥcan inhibit vicious cycle of degeneration of collagen in the extracellul

关 键 词：川续断皂苷Ⅵ 跟腱病 修复 胶原 细胞外基质 肌腱细胞 增殖 纤维化 

分 类 号：R453[医药卫生—治疗学] R685[医药卫生—临床医学]