机构地区:[1]南方医科大学中西医结合医院,广东省广州市510315 [2]南方医科大学中医药学院,广东省广州市510515
出 处:《中国组织工程研究》2022年第2期225-231,共7页Chinese Journal of Tissue Engineering Research
基 金:国家自然科学基金(81674048),项目负责人:周国平。
摘 要:背景:既往电针对大鼠脑缺血再灌注损伤保护作用及时效性的机制研究较少。目的:观察不同时间电针对脑缺血再灌注大鼠MAPK家族3条信号通路中p-ERK、p-JNK及p-p38MAPK蛋白表达的影响,探索电针发挥脑保护作用的具体机制。方法:150只SD雄性大鼠随机分为假手术组、模型组、电针组(后2组线栓法制备右侧大脑中动脉栓塞缺血模型),每组50只,每组再设脑缺血1.5 h后再灌注2 h、6 h、1 d、3 d、7 d共5个时间亚组,每亚组10只。电针各亚组在规定时间点行单次电针治疗,选取"合谷""尺泽""足三里""三阴交",予疏密波,频率2 Hz,强度1 mA,治疗20 min;电针的同一时间抓取模型组和假手术组的大鼠进行固定,但不进行电针干预。在相应时间点进行神经功能缺损评分,之后大鼠麻醉后取脑,TTC染色法观察脑梗死面积,免疫荧光双标法结合检测p-ERK、p-JNK、p-p38MAPK的表达。结果与结论:(1)与假手术组相比,模型组同时段各亚组神经功能缺损评分升高、脑梗死体积增大(P<0.05);与模型组相比,电针组再灌注6 h、1 d、3 d亚组神经功能缺损评分显著降低、脑梗死体积明显减少(P<0.05);(2)与模型组比较,电针组再灌注1,3 d出现p-ERK表达的明显上调(P<0.05)及p-JNK的表达显著下降(P<0.05),p-p38MAPK在再灌注6 h、3 d时下降明显再灌注;(3)结果说明电针可减少脑梗死体积,减轻神经功能缺损,并上调p-ERK同时下调p-JNK、p-p38MAPK的表达,且电针在再灌注6 h-3 d内效果最佳;电针促进缺血再灌注引起的脑组织损伤修复,通过调控MAPK家族信号通路发挥脑保护作用。BACKGROUND:The mechanisms underlying the protection of electroacupuncture against cerebral ischemia/reperfusion injury in rats and its temporal efficacy have been less studied in the past.OBJECTIVE:To observe the effect of electroacupuncture on the expression of p-ERK,p-JNK and p-p38MAPK proteins in three signaling pathways of the MAPK family in rats with cerebral ischemia/reperfusion at different times,and to explore the specific mechanism underlying the cerebral protection of electroacupuncture.METHODS:150 Sprague-Dawley male rats were randomly divided into sham-operated group,model group,and electroacupuncture group,with 50 rats in each group.Each group was then set up for ischemia 1.5 hours followed by reperfusion for 2 hours,6 hours,1 day,3 days,and 7 days,with 10 rats in each subgroup.Single electroacupuncture treatment at Hegu,Shakuze,Zusanli,and Sanyinjiao was conducted at the given time,giving sparse waves at a frequency of 2 Hz,intensity of 1 mA,20 minutes for one session.Rats in the model and sham-operated groups were grasped simultaneously for fixation at the same time of electroacupuncture,but no electroacupuncture intervention was performed.After anesthesia,the brains were decapitated at the corresponding time points,and neurological deficit scoring was used to observe the infarct area by TTC staining.The expression of p-ERK,p-JNK,and p-p38MAPK was detected by double immunofluorescence labeling method.RESULTS AND CONCLUSION:Compared with the sham-operated group,the model group had higher neurological deficit scores and larger infarct size in each subgroup in the same period(P<0.05).Compared with the model group,there were significantly lower neurobehavioral scores and smaller infarct size in the electroacupuncture subgroups of 6 hours,1 day and 3 days(P<0.05).Compared with the model group,the electroacupuncture group had significant upregulation of p-ERK expression at 1 and 3 days of reperfusion(P<0.05),downregulation of p-JNK expression at 1 and 3 days of reperfusion(P<0.05),and significant downreg
分 类 号:R318[医药卫生—生物医学工程] R459.9[医药卫生—基础医学]
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